OBJECTIVE To determine whether acellular human dermis is degraded by matrix metalloproteinases (MMPs), a large class of matrix-degrading enzymes. METHODS The degradation of acellular human dermis specimens was evaluated in vitro. Wild-type murine fibroblasts with a broad-spectrum MMP inhibitor, GM6001, and MMP-2-deficient fibroblasts were placed on the basement membrane and dermal surfaces of...

Previous studies showed that several miRNAs can regulate pathways involved in UVB-induced premature senescence and response to ultraviolet irradiation. It has also been reported that miR-34c-5p may be involved in senescence-related mechanisms. We propose that miR-34c-5p may play a crucial role in senescence of normal human primary dermal fibroblasts. Here, we explored the roles of miR-34c-5p in...

Skin photodamage, which is induced by ultraviolet (UV) radiation, a prevalent cause of skin damage. In this study, transdermal drug delivery system developed for the topical treatment composed tetrahedral framework nucleic acids (tFNAs) and lipoic acid (LA). The tFNAs-LA (TLA) nanocomposite exhibits excellent biocompatibility, as well antioxidant, anti-apoptotic, anti-inflammatory capabilities....

BACKGROUND Despite numerous treatments available for deteriorated cutaneous wound healing such as a diabetic foot, there is still the need for more effective therapy. Adipose-derived stem cells (ASCs) are mesenchymal stem cells, which are self-renewing and multipotent. Mesenchymal stem cells have the potential for tissue repair and regeneration. OBJECTIVE To investigate the effects of human A...

Extracts from Saccharomyces cerevisiae are incorporated in a lot of cosmetic products on the market, but benefits extracts lack scientific reports. We tested toxicity and anti-senescent activity an extract S. vitro model, human dermal fibroblast (HDF) cell culture. chronicled development extraction method subsequent biochemical assays. used two methods which were hot water rapid spin. The optim...

In this study, we have investigated the proliferative response of human dermal fibroblasts isolated from normal and psoriatic individuals to different cytokines. We found that IL-1, TNF (α) and EGF are stimulatory whereas IFN « and 1 both showed inhibitory activity on fibroblast proliferation. IL-6 a more recently described cytokine, showed no detectable effects on cell proliferation. Inve...

Background: Dermal fibroblast is a powerful tool for the study of ex vivo DNA delivery in development of both cell therapy and tissue engineering products. Using genetic modification, fibroblasts can be diversely adapted and made suitable for clinical gene therapy. In this study, we first compared several non-viral transfection methods including nucleofection in rat and human primary dermal fib...

The repair strategy for the healing of skin wounds in fetuses differs from that in adults. Proliferation and migration of dermal fibroblasts are the main mechanisms associated with skin wound healing, as well as the complex interactions between epidermal keratinocytes (KCs) and dermal fibroblasts. In order to investigate the effects of fetal skin epidermal KCs on fetal and adult human dermal fi...

The aim of this study is to investigate the applicability of poly(lactic-co-glycolic acid) (PLGA)/collagen composite scaffold for skin tissue engineering. PLGA and collagen were dissolved in HFIP as a common solvent and fibrous scaffolds were prepared by electrospinning method. The scaffolds were characterized by scanning electron microscopy (SEM), FTIR spectroscopy, mercury porosimetry, tensil...

Cell membranes prepared from senescent human diploid fibroblasts (HDF) inhibited entry into S phase by 35% when added to the medium of replicating young HDF. This membrane-associated inhibitory activity was (i) sensitive to trypsin, heat, and periodate, which suggests that the inhibitor is a glycoprotein, and (ii) not able to inhibit DNA synthesis in simian virus 40-transformed HDF, which indic...