We have previously demonstrated that the kynurenine pathway (KP), the major biochemical pathway for tryptophan metabolism, is dysregulated in many inflammatory disorders that are often associated with sexual dimorphisms. We aimed to identify a potential functional interaction between the KP and gonadal hormones. We have treated primary human macrophages with progesterone in the presence and abs...

β2m: β2-Microglobulin; β2m-free HC: β2m-free Heavy Chain; CTL: CD8+ Cytotoxic T Lymphocytes; DSA: Donor Specific Antibodies; HC: Heavy Chain Polypeptide; HLA: Human Leukocyte Antigens; HLA-I: Human Leukocyte Antigen Class I; HLA-II: Human Leukocyte Antigen Class II; IFNγ: Interferon-γ; kDa: kilodalton; MHC: Major Histocompatibility Complex; MMP: Matrix Metalloproteinase; rHLA: Recombinant HLA; ...

Tuning recombinant protein expression is an approach which can be successfully employed for increasing the yield of recombinant protein production in high cell density cultures. On the other hand, most of the previous results reported the optimization induction conditions during batch and continuous culture of recombinant E. coli, and consequently fed-batch culture have received less attention....

background:the premature termination of high producer clones, which will be killed due to cell proliferation and proteins production antagonism, is one of the basic drawback in recombinant proteins technology. furtheremore, it is supposed some toxic proteins like interferon which we intended to clone and express, inhibit host cells’ proliferation. so, it is necessary to tightly control ifn-γ pr...