A civil paternity investigation involving the parents of deceased alleged father in order to establish a family relationship is presented. On basis 23 autosomal short tandem repeat (aSTR) genotyping results, conclusive proof was not achieved, as probability (W) calculated 0.99988. Additional genetic data 17 classical and non-classical human leukocyte alleles (HLA) typing by next-generation sequ...

BACKGROUND Epitopes are the sites to which antibodies bind. Both alpha and beta peptide chains of the human leukocyte antigen-DQ heterodimers (DQA1 and DQB1, respectively) contain polymorphic regions. We can identify DQA1 and DQB1 epitopes by DQ single antigen beads assay of the antibodies, correlating the beads' reaction patterns with either DQA1 or DQB1 alleles. METHODS Sera from 74 transpl...

Coeliac disease is an inflammation of the small intestine, occurring in genetically susceptible individuals triggered by the ingestion of gluten. Human Leukocyte Antigens (HLA) DQ2 and DQ8 gene have been identified as key genetic factors in coeliac disease as they are presented in almost 100 % of the patients. These genes are encoded by the combination of certain alleles in the DQA and DQB regi...

OBJECTIVES Although previous studies have investigated the effect of human leukocyte antigen matching on long-term outcomes after heart transplants, its role in the prognosis after a heart transplant remains unclear, particularly with respect to short-term survival. MATERIALS AND METHODS We evaluated the human leukocyte antigen mismatch on in-hospital mortality of 158 consecutive patients who...

Abstract A major technical hurdle for T cell immune profiling is the time and cost to accurately genotype Human Leukocyte Antigen (HLA) loci from peripheral blood. Here, we developed a rapid, highly multiplexed approach HLA typing using RNA <100,000 blood mononuclear cells with Oxford Nanopore Technology (ONT) Minion sequencer. This method uses selective reverse transcription of mRNA six...