background: rapidly increasing possibilities of exposure to environmental extremely low-frequency electromagnetic fields (elf-emf) have become a topic of worldwide investigation. epidemiological and laboratory studies suggest that exposure to elf-emf may increase cancer risk therefore assessment of chromosomal damage in various cell lines might be of predictive value for future risk estimation....

Tumor-specificity (TS) and anti-inflammatory activity of benzo[b]cyclohept[e][1,4]oxazin-6(11H)-one, generally known as benzoxazinotropone (BOT), have been reported. In order to find a new biological activity, the combination effect of BOT and three apoptosis-inducing agents was investigated. Cytotoxicity against four human oral squamous cell carcinoma (OSCC) cell lines and five human oral norm...

objective: in necrotic immature teeth, intra canal medicaments such as triple antibiotic paste (tap) and calcium hydroxide (ch) are used for root canal disinfection and regeneration treatment. however, the effect of these medicaments on dental pulp fibroblasts has yet to be known. this study aimed to assess the cytotoxicity of ch and tap against cultured human dental pulp fibroblasts (hdpfs) ob...

Tissue engineering of bone and oral mucosa have been extensively studied independently. The aim of this study was to develop and investigate a novel combination of bone and oral mucosa in a single 3D in vitro composite tissue mimicking the natural structure of alveolar bone with an overlying oral mucosa. Rat osteosarcoma (ROS) cells were seeded into a hydroxyapatite/tri-calcium phosphate scaffo...

Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), and M. K. Cook. Suppression of Rous sarcoma virus growth in tissue cultures by Mycoplasma orale. J. Bacteriol. 90:534-540. 1965.-An agent which produced cell destruction in human diploid and chick-embryo fibroblasts was isolated from WI-26 strain of human diploid fibroblasts and shown to be a mycoplasma....

introduction: oral manifestations can be the first signs of human immunodeficiency virus/acquired immunodeficiency syndrome (hiv/aids) and a useful marker for the progression of this disease. the present study aimed to determine the prevalence of oral manifestations and examine their relationship with socio-demographic factors in hiv-positive patients in the health centers affiliated to zahedan...

Objective: Kaempferol is a natural flavonol found in many dietary fruits, vegetables, and medicinal herbs. It reportedly has multiple beneficial properties as analyzed in vivo and in vitro. The objective of this study was to determine cytoprotective, anti-inflammatory, and wound healing characteristics of kaempferol in cultured human gingival fibroblasts. Materials & Methods: To determine cytot...

Khat chewing is widely practiced in Eastern Africa and the Middle East. Khat is genotoxic to cells within the oral mucosa, and several studies have suggested an association between khat use and oral lesions like hyperkeratosis and oral cancer. This study investigated the mechanism of khat-induced cytotoxicity using primary normal human oral keratinocytes (NOK) and fibroblasts (NOF). Khat induce...

Phytochemical analysis of the rhizomes of Cimicifuga racemosa (Ranunculaceae) resulted in the isolation of twelve cycloartane glycosides (1--12), including four new ones (4--6, 12). The structures of the new compounds were determined by spectroscopic analysis, including two-dimensional (2D) NMR data, and chemical methods. The isolated compounds were evaluated for their cytotoxic activities agai...

We have studied the effects of the oral sulfonylurea agent glyburide to modulate insulin receptors on nontransformed human fibroblasts in tissue culture. When glyburide was added to monolayers of human fibroblasts, a dose-dependent increase in the number of cell surface receptors was observed with a maximum effect (19% increase) seen at 1 microgram/ml glyburide. Insulin can induce a loss of ins...