Seventy-four cultures of Pasteurella multocida representing all four capsular types, A, B, D, and C, from various animal species and diseases were examined for the production of hyaluronidase by two procedures. In one, hyaluronidase production was determined by the depolymerization of streptococcal capsular hyaluronic acid, and in the other, production was determined by degradation of sodium hy...

A comparative study of spreading factor and hyaluronidase in preparations from various sources revealed the following points of similarity and dissimilarity in the two reactions. 1. Similarities: (a) All preparations containing hyaluronidase also produced spreading. (b) Heating at 65 degrees and 100 degrees C. for 30 minutes produced a comparable effect on both reactions. (c) The demonstration ...

OBJECTIVE Functional studies show that disruption of endothelial surface layer (ESL) is accompanied by enhanced sensitivity of the vasculature towards atherogenic stimuli. However, relevance of ESL disruption as causal mechanism for vascular dysfunction remains to be demonstrated. We examined if loss of ESL through enzymatic degradation would affect vascular barrier properties in an atherogenic...

Embryonic chick chondrocytes were cultured in the presence or absence of different preparations of testicular hyaluronidase. This treatment inhibited the accumulation of cartilage matrix as indicated by phase-contrast microscopy, by Alcian green staining, and by accumulation of 35S-labeled material. In addition, most preparations of testicular hyaluronidase caused a conversion of the cells to a...

One hundred and seven (41%) of 262 isolates of Streptococcus milleri, from human sources, produced hyaluronidase. Hyaluronidase production was commoner in beta haemolytic isolates 32 of 39 (82%), many of which were of Lancefield group F. But hyaluronidase was also found in alpha and non-haemolytic isolates, and in groups A, C, G, and non-groupable isolates. There was a strong association betwee...

The administration of hyaluronidase is a promising intervention to protect the ischemic myocardium in man, but evidence of the extent to which it may reduce the ultimate size of an infarct is not well-defined. Hence, open chest, anesthetized dogs were randomized into 10 control dogs which received saline and eight treated dogs which received three doses of hyaluronidase (500 NF units/kg I.V.) a...

In view of the evidence which has been presented that hyaluronidase is not inhibited by salicylates in therapeutic concentrations, it would seem that Guerra's explanation of the anti-rheumatic action of these drugs in terms of hyaluronidase inhibition must be abandoned. The question naturally arises as to whether or no their apparent anti-histamine action can be invoked in this connexion, as we...

myces. Biochim Biophys Acta 198: 607-609 Orkin RW, Toole BP (1978) Hyaluronidase activity and hyaluronate content of the developing chick embryo heart. Dev Biol 66: 308-320 Polansky JR, Toole BP, Gross J (1974) Brain hyaluronidase: Changes in activity during chick development. Science 183: 862-864 Ressig JL, Strominger JL, LeLoir Lf (1955) A modified colorimetric method for the estimation of N-...

BACKGROUND The enzyme hyaluronidase from bovine origin is commonly used for oocyte-cumulus cell removal in ICSI. A recombinant human hyaluronidase (rHuPH20) has been introduced as a quality-controlled and safe alternative. METHODS In order to validate its effectiveness, a non-inferiority trial was started on sibling cumulus-oocyte complexes (135 ICSI patients). Oocyte denudation involved enzy...