The use of novel intercalating dyes as labels in DNA restriction fragment analysis by capillary electrophoresis with frequency-domain fluorescence lifetime detection is described. The dyes, including one mono-intercalating dye with three positive charges and three bis-intercalating, homodimeric dyes with four positive charges, were excited by the 488 nm line of an argon ion laser and exhibited ...

Purnose The incorporation of polymers into artificial tears both increases the thickness of the precorneal tear film and decreases the rate of drainaae of the for8nulations. On the basis of these effects, we hypothesized that a time study of the artificial increase in tear volume induced by such formulations could be a useful method of assessing their precorneal residence time. Methods New Zeal...

Purnose The incorporation of polymers into artificial tears both increases the thickness of the precorneal tear film and decreases the rate of drainaae of the for8nulations. On the basis of these effects, we hypothesized that a time study of the artificial increase in tear volume induced by such formulations could be a useful method of assessing their precorneal residence time. Methods New Zeal...

A Multilayered Multidisk Tablet (MLMDT) comprising two drug-loaded disks enveloped by three drug-free barrier layers was developed for use in chronotherapeutic disorders, employing two model drugs, theophylline and diltiazem HCl. The MLMDT was designed to achieve two pulses of drug release separated by a lag phase. The polymer disk comprised hydroxyethylcellulose (HEC) and ethylcellulose (EC) g...

Microbicides are considered a promising strategy for preventing human immunodeficiency virus (HIV-1) transmission and disease. In this report, we first analyzed the antiviral activity of the miniCD4 M48U1 peptide formulated in hydroxyethylcellulose (HEC) hydrogel in activated peripheral blood mononuclear cells (PBMCs) infected with R5- and X4-tropic HIV-1 strains. The results demonstrate that M...

Understanding nanoparticle diffusion within non-Newtonian biological and synthetic fluids is essential in designing novel formulations (e.g., nanomedicines for drug delivery, shampoos, lotions, coatings, paints, etc.), but is presently poorly defined. This study reports the diffusion of thiolated and PEGylated silica nanoparticles, characterized by small-angle neutron scattering, in solutions o...

Václavíková E., Kvasnička F. (2013): Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements. Czech J. Food Sci., 31: 55–65. Glucosamine and chondroitin sulfate, components of normal cartilage, are used as ingredients in dietary supplements intended to treat osteoarthritis and/or to support joint health. Of concern is the documented lack of quality in many ...

The aim of the study was to produce a model form of a drug of anti-inflammatory activity designed for application in oral cavity. An attempt was made to choose hydrogel vehicle which would ensure not only high pharmaceutical availability of the therapeutic agent but appropriate theological properties of the drug as well. Variant prescriptions with ketoprofen were worked out which differed in th...