35 unrelated individuals were studied for characterization as either heterozygous or homozygous for beta-thalassemia. Molecular analysis was done by PCR/RFLP to detect the mutations most commonly associated with beta-thalassemia (β(0)IVS-I-1, β(+)IVS-I-6, and β(0)39). In the patients who showed none of these mutations, the beta-globin genes were sequenced. Of the 31 heterozygous patients, 13 (4...

baluchi sheep is considered as the most common breed in iran, constituting about 30% of total sheep population, approximately 15 million heads. this research was designed to study the incidence of mutation in two loci of igf-i (exon 3) and adrb3 (intron) along with their association with body weight traits in baluchi sheep population. following dna extraction from 190 baluchi sheep, two pairs o...

background: β-thalassemia is a common autosomal recessive disorder resulting from over 200 different mutations of beta glo­bin genes. the aim of the present study was to identify the distribution and frequency of the most com­mon β-thalassemia mu­tations among the population of isfahan province in central iran. methods: the data presented here were derived from a total of 114 β-thalassemia chro...

In most bioluminescence systems the oxidation of luciferin and production of light is catalyzed by luciferases. Protein engineering studies have shown that thermostable proteins from thermophilic organisms have a higher frequency of Arg, especially in exposed states. To further clarify the arginine saturation effect on thermostability of firefly luciferase, some of hydrophobic solvent-exposed r...

Some hepatocellular adenoma (HCA) subtypes are characterized by different CTNNB1 mutations, leading to beta-catenin activation levels, hence variable immunostaining patterns of glutamine synthetase (GS) expression, and risks malignant transformation. In a retrospective multicentric study 63 resected inflammatory (n=33) noninflammatory (n=30) molecularly confirmed CTNNB1-mutated b-(I)HCA, we inv...