This study characterizes the glucomannan utilization operon (gmuBACDREFG, formerly ydhMNOPQRST) of Bacillus subtilis. Transcription of the operon is induced by konjac glucomannan and requires the last mannanase gene (gmuG). Cellobiose and mannobiose, possible degradation products of glucomannan by GmuG, are strong inducers of transcription. It is shown that an internal regulator gene (gmuR) enc...

An operon is a functioning unit of genomic DNA containing a cluster of genes under the control of a single promoter. Gene regulation is achieved by the control of an operon through induction or repression. Operons are of two types: inducible operons and repressible operons. Inducible operon is a type of operon which gets switched on by a substrate chemical, i.e., an inducer. In a repressible op...

The ethanolamine utilization (eut) operon of Salmonella typhimurium is controlled by a positive regulatory protein (EutR) which stimulates eut operon expression in response to the simultaneous presence of two effectors, ethanolamine and adenosyl-cobalamin (Ado-B12). Ado-B12 is a cofactor for ethanolamine ammonia-lyase (lyase), the first enzyme in the ethanolamine-degradative pathway. The depend...

Streptomyces coelicoloris a non-pathogenic soil saprophytic bacterium and is model organism for antibiotic production. This species contains single copy of nine gene cluster known as the mammalian cell entry (mce) operon. operon was originally characterised in Mycobacterium tuberculosisas an important virulence factor acting invasion survival within macrophages encodes ABC transporter cholester...

Proteomic analysis is a highly effective method for bacteria identification and the elucidation of protein's content in prokaryotic cells at different growth conditions. To our knowledge this approach hardly ever used characterization producers biologically active substances. The understanding changes protein profile mutant strains capable substances overproduction helps to recognize biochemica...

The vanE operon was characterized from Enterococcus faecalis N00-410 (MIC of vancomycin = 24 microg/ml). The organization of the vanE operon was identical to that of the vanC1 operon from Enterococcus gallinarum, with protein identities ranging from 46 to 63%. An open reading frame located downstream of the vanE operon showed significant homology to a number of integrase genes, all of which are...

We predict the operon structure of the Bacillus subtilis genome using the average operon length, the distance between genes in base pairs, and the similarity in gene expression measured in time course and gene disruptant experiments. By expressing the operon prediction for each method as a Bayesian probability, we are able to combine the four prediction methods into a Bayesian classifier in a s...

This article presents evidence that starvation for leucine in an Escherichia coli auxotroph triggers metabolic activities that specifically target the leu operon for derepression, increased rates of transcription, and mutation. Derepression of the leu operon was a prerequisite for its activation by the signal nucleotide, guanosine tetraphosphate, which accumulates in response to nutritional str...

BACKGROUND In an effort to identify alternate recombinant gene expression systems in Pseudomonas fluorescens, we identified genes encoding two native metabolic pathways that were inducible with inexpensive compounds: the anthranilate operon (antABC) and the benzoate operon (benABCD). RESULTS The antABC and benABCD operons were identified by homology to the Acinetobacter sp. anthranilate opero...