Intravenous immunoglobulin (lVlgG) causes an acute rise in the platelet count in the majority of patients with chronic immune thrombocytopenic purpura (ITP) but the mechanism(s) of action is still unknown. We evaluated the ability of three different IVIgG preparations to inhibit the in vitro binding of autoantibody to platelet glycoprotein (GP) llb/ lila. ITP plasma. known to contain anti-GPIIb...

Following stimulation with adenosine diphosphate (ADP). collagen. or arachidonic acid. unstirred human platelet suspensions bind ‘25l-fibrinogen in a reaction that reaches completion within 30 mm. Scatchard analysis of these binding data reveals two sets of binding sites with all 3 agents: a high affinity site (Kd 0.029-0.045 MM) binding 1 000-i 600 fibrinogen molecules per platelet. and a lowe...

Objectives: Cultured skin substitutes (CSSs), consisting of human keratinocytes and human fibroblasts attached to collagen-glycosaminoglycan substrates, have been demonstrated to cover wounds, and may release detectable quantities of growth factors that promote wound healing. Materials and Methods: Basic fibroblast growth factor (bFGF), interleukin-la (IL-la), and interleukin-6 (IL-6) were assa...

Antibodies that bind to human platelet membrane glycoproteins llb and lIla were used to develop methods for analyzing platelet membrane components by flow cytometry. Platelets were tentatively identified by their lowintensity light scatter profiles in whole blood or plateletrich plasma preparations. Identification of this cell population as platelets was verified by using plateletspecific antib...

El projecte d’eixample d’Ildefons Cerdà per al pla de Barcelona proposava un traçat il·limitat basat en una retícula moderna carrers amplis i grans illes edificades. Una característica del era l’homogeneïtat la forma urbana. Factors com l’activitat agrícola prèvia, però, van condicionar el creixement l’Eixample dos teixits urbans diferenciats. D’una banda, es fa referència a l’eixample central,...

The platelet retention test provides a measure of the number of platelets retained in a column of glass beads and is one of the few in vitro platelet function tests that is abnormal in von Willebrand’s disease (vWd). In a two-stage test, 1 mL of blood (designated A) was passed through the column, followed by 5 mL of isotonic saline and then 5 mL of blood (B) in which platelet retention was meas...