introduction: prostate specific antigen (psa) is one of the most sensitive markers for diagnosis of prostate cancer. immunoradiometric kit (irma) is a common and sensitive method for determination of psa in clinical laboratories. this kit has four major components: solid phase coated with monocolonal antibody, pair antibody labelled with i-125, series of standards in different ranges of concent...

BACKGROUND Since the introduction of the first radioimmunoassay, several improvements have been made in the design of immunoassays such as method of antibody production, labeling, automation and detection technology. We performed an analytical evaluation of the new electrochemiluminescent immunoassay (ECLIA) for serum TSH, FT4 and T3 in the Elecsys 2010 immunoassay system and compared the resul...

Prostate cancer is the sixth most common cancer in the world, the third most common cancer in men. In 2000, the number of new cases of prostate cancer was estimated at 513000 worldwide and this disease accounts for 9.7% of all cancers in men (1, 2). Thus early detection and local treatment have been advocated in an effort to influence the significant morbidity and mortality associated with the ...

Commercial kits give different measurements for concentrations of growth hormone (GH, somatotropin) in serum. Most notably, a two-site monoclonal-antibody-based immunoradiometric assay (IRMA) from Hybritech routinely yields lower values than do conventional RIAs in which polyclonal antibodies are used. We used purified dimeric biosynthetic human GH as a model compound to investigate the specifi...

A cross-sectional study of thyrotropin (TSH), thyroxine (T4) and triiodothyronine (T3) in human serum was performed aiming to define their normal values. In total, 201 assays were performed on 70 normal individuals, 26 men and 44 women, with ages ranging from 2 to 84 years. Serum TSH was measured with a highly sensitive immunoradiometric assay (IRMA) kit and T4 & T3 were determined with commerc...

This paper describes the results of the information modelling work group from the International Workshop on Models For Computer-Integrated Construction in Finland, October 1992. At this workshop, researchers from around the world presented their individual modelling efforts. These models’ parallel goals and abundant similarities led the participants to combine their individual results into a si...

IRMA is a set of multimedia and software tools implemented to encourage the dissemination of scientific culture. On the basis of web technologies we have developed an environment to help learner approaching some mathematical topics, which become accessible through historical news, simulation and interactive applications. In this paper, we will explore the main design and implementation issues o...

Newborn Screening for the diagnosis of Congenital Hypothyroidism (CH) was first started in Bangladesh 1999 by Immunoradiometric assay (IRMA). Dissociation enhanced lanthanide fluorescent immunoassay (DELFIA) 1986 due to its superior sensitivity, speed, and non-radioactive methodology. Auto DELFIA 1235 automatic system introduced In vitro Division National Institute Nuclear Medicine & Allied...

BACKGROUND Serum thyroglobulin (Tg) is essential in the follow-up of patients with differentiated thyroid carcinoma (DTC). However, interchangeability and standardization between Tg assays have not yet been achieved, even with the development of an international Tg standard (Certified Reference Material 457 [CRM-457]). METHODS Serum Tg from 30 DTC patients and serially diluted CRM-457 were me...