Current vaccination strategies for commercial poultry using live attenuated and inactivated Newcastle disease (ND) vaccines have some limitation and difficulties, and new vaccines with distinct features are needed. Recently, in ovo vaccination technology is concerned as a safe, efficacious, and convenient method. Common ND vaccines used in chickens cannot be employed in ovo due to embryo toxici...

The present study was carried out in co-operation between the Poultry Production Unit, Nuclear Research Center, Atomic Energy Authority, Cairo, Egypt and Ostrich Farm belonging to Faculty of Agriculture, Al-Azhar University. In an effort to improve the hatchability and hatching performance of ostrich eggs via testing the effect of in ovo injection of several nutrients and vitamins, as no simila...

The Drosophila ovo gene, which encodes a putative transcription factor (Ovo) with TFIIIA-like zinc fingers, is required for female germline survival and proper oogenesis. Three dominant female-sterile ovoD mutations cause ovarian abnormalities that define an allelic series, with ovoD1 displaying the stronger phenotype and ovoD3 the weaker. We report here that all three ovoD mutations are point ...

probiotics are beneficial microorganisms which will be considered as alternatives to antibiotic growth promoters.the aim of the present study was to investigate the effect of probiotic administration in hatchery on performance, blood parameters and immune response of broilers. three hundereds 1-d-old male chicks (ross 308) were assigned to five experimental groups of three replications. birds o...

Chicken embryonic retina is an excellent tool to study retinal development in higher vertebrates. Because of large size and external development, it is comparatively very easy to manipulate the chick embryonic retina using recombinant DNA/RNA technology. Electroporation of DNA/RNA constructs into the embryonic retina have a great advantage to study gene regulation in retinal stem/progenitor cel...

Two experiments were conducted to standardize in ovo injection of amino acids (AA) and to evaluate the effect of in ovo injection of limiting AA(s) on pre and post hatch growth performance, immune response and development of digestive organs. Combinations of essential and non-essential amino acids (Lys+Arg, Lys+Met+Cys, Thr+Gly+Ser, Ile+Leu+Val and Gly+Pro) were injected into 50 eggs in each tr...

The location of injection and vaccine deposition in ovo is known to be critical to the efficacy of Marek's disease (MD) vaccine protection against MD viral challenge. Vaccine deposition into the amniotic sac or a s.c. or i.m. site of the embryo is required for MD vaccine efficacy. Vaccine deposition into the air cell or allantoic fluid results in chicks that are not adequately protected against...