نتایج جستجو برای: in silico cloning
تعداد نتایج: 16991398 فیلتر نتایج به سال:
The rapid expansion of nucleotide sequence data available in public databases is revolutionizing biomedical research. These databases have a variety of uses, including the discovery of novel genes, identification of homologous genes, analysis of alternative splicing, chromosomal localization of genes, and detection of polymorphisms. Data sets such as the human transcript map will undoubtedly ac...
As genome of B. mori is available in GenBank, identification of novel genes of B. mori can be carried out. In this study, we used the in silico cloning method to obtain the Glucuronyltransferase-S (GlcAT-S) gene of B. mori and analysed with bioinformatics tools. The result was confirmed by RT-PCR,prokaryotic expression and western blot. The GlcAT-S cDNA contains a 843bp ORF and has three exons....
In this work, we reported the synthesis and evaluation of antimycobacterial and antifungalactivity of a series of thiocarbohydrazone derivatives which are thiacetazone congeners. Thetarget compounds were synthesized in superior yields by reacting thiocarbohydrazide withdifferent aromatic aldehydes and methyl ketones. Compounds 8, 19 and 25 were found to bethe most potent derivatives, exhibiting...
The normalization and subtraction of highly expressed cDNAs from relatively large tissues before cloning dramatically enhanced the gene discovery by sequencing for the mouse full-length cDNA encyclopedia, but these methods have not been suitable for limited RNA materials. To normalize and subtract full-length cDNA libraries derived from limited quantities of total RNA, here we report a method t...
Introduction: Cloning technology has been strengthened a long human desire for eternal life. The possibility of human cloning is the new challenges in medical education and medical ethics. The present study discussed the different perspectives in this field. Methods: This study is a review article. Related books and some database such as SID, Irandoc, Elsevier, PubMed, Magiran were searched us...
پس از سال ها تلاش برای پیشگیری، تشخیص و درمان عفونت hbv، هنوز این عفونت به صورت یک تحدید سلامتی در سراسر دنیا مطرح می باشد. این میزان تحدید با وجود حدود 400 میلیون ناقل مزمن برای عفونت و مرگ ومیر سالانه یک میلیون نفر از این عفونت رنگ بیشتری را به خود می گیرد. به سبب این میزان از اهمیت یافتن راه های موثرتر تشخیص این عفونت از اهمیت زیادی برای شناسایی افراد مبتلا برخوردار خواهد بود. برای نیل به ای...
باکتری clostridium perfringens (c. perfringens) یک باسیل گرم مثبتِ بی هوازی هاگ دار و بیماری زا است که در طبیعت، و سیستم گوارش و برخی دیگر از اندام های بدن انسان و دام، به وفور یافت می شود. توکسین های اپسیلون و بتا از توکسین های اصلی c. perfringens تیپ هایd وb هستند که از فاکتورهای ویرولانس در این تیپ ها بوده و عامل ایجاد بیماری های انتریت نکروزی، انتروتوکسمی و قلوه نرمی در گوسفند، بز و بندرت در...
lactoferrin (lf) is an iron-binding multi-functional glycoprotein which has numerous physiological functions such as iron transportation, anti-microbial activity and immune response. in this study, different in silico approaches were exploited to investigate lf protein properties in a number of mammalian species. results showed that the iron-binding site, dna and rna-binding sites, signal pepti...
Human cloning is one of the latest and most complicated developments in the field of medical and genetic sciences that could fulfill man’s dream of eternal life. Scientists’ achievements in this respect, however, have brought about many concerns for thinkers, particularly ethics scholars. Human and animal cloning have been examined from different angles, but one basic aspect of human cloning ...
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