Phosphoenolpyruvate carboxylase (PEPC) purified from maize (Zea mays L.) leaves associates with maize leaf chloroplast membrane in vitro. The binding of PEPC to the membrane results in enzyme inactivation. A protein isolated from a maize leaf chloroplast membrane preparation inactivated PEPC. Treatment with membrane preparation or with partially purified inactivating protein accelerates PEPC in...

Bradyrhizobium ORS285 forms a nitrogen-fixating symbiosis with both Nod factor (NF)-dependent and NF-independent Aeschynomene spp. The ribBA gene encodes for putative bifunctional enzyme 3,4-dihydroxybutanone phosphate (3,4-DHBP) synthase guanosine triphosphate (GTP) cyclohydrolase II activities, catalyzing the initial steps in riboflavin biosynthesis pathway. In this study, we show that inacti...

It has been known for many years that for a "normal" un-transformed cell to become immortal and subsequently tumorigenic requires multiple pro-oncogenic changes in the levels of protein expression and function. Genes most commonly associated with the process of oncogenesis include: p53 inactivating mutation; hDM2 overexpression; p16 reduced expression; K-/H-RAS activating mutation; PTEN inactiv...

The voltage-dependent calcium current (ICa) in cultured human neuroblastoma cells (NB-I) was studied by whole-cell recording. The low-threshold current (Il), the high-threshold, fast inactivating current (Ih.f.), and the high-threshold, slow inactivating current (Ih.s.) were identified. Il. was blocked by Ni2+. Ih.f. was blocked by omega-conotoxin GVIA. Ih.s. was blocked by nifedipine, and enha...

Special high-protein foods suitable for diabetics must be treated to ensure the complete absence of microorganisms and bacteria. It is also important achieve that this treatment does not change nutritional value product. Among new decontamination technologies, low-energy electron-beam has proven an effective technique inactivating bacteria with minimal impact on food quality. The paper aims ana...

Large-conductance Ca2+- and voltage-dependent potassium (BK) channels exhibit functional diversity not explained by known splice variants of the single Slo alpha-subunit. Here we describe an accessory subunit (beta3) with homology to other beta-subunits of BK channels that confers inactivation when it is coexpressed with Slo. Message encoding the beta3 subunit is found in rat insulinoma tumor (...