introduction: anthroponotic cutaneous leishmaniasis (acl) is still a major public health problem in the northeast and central parts of iran. this study was designed to compare microscopy and cultivation methods with pcr amplification of kinetoplast dna and its1 followed by rflp analysis for diagnosis of acute and chronic acl. methods: in this study, 66 patients with acl including 24 acute and 4...

The 16S-23S rRNA gene internal transcribed spacer region (ITS1) from 34 strains of Pseudomonas avellanae and some strains of Pseudomonas syringae pathovars was amplified and assessed by restriction fragment length polymorphism (RFLP) using 10 restriction enzymes. In addition, the ITS1 region of four representative P. avellanae strains was sequenced and compared by the neighbour-joining algorith...

Ants of the genus Formica play an important role in biogenesis by participating various processes, including formation complex trophic networks. The ants ecosystem depends on their species and geographic population, which can be difficult to identify. gagatoides with a wide range F. kozlovi are among some examples. question is whether Siberian populations really belong this or local gagatoides....

Plant parasitic nematode genera commonly found associated with sugarcane were collected from trial sites in various regions of the sugar industry. Nucleic acids were extracted and the rDNA internal transcribed spacer 1 (ITS1) region amplified. Identification of the important genera was possible by size differentiation of the amplification products. Sequencing of the ITS1 region was used to conf...

Anaerobic fungi are key players in the breakdown of fibrous plant material in the rumen, but not much is known about the composition and stability of fungal communities in ruminants. We analyzed anaerobic fungi in 53 rumen samples from farmed sheep (4 different flocks), cattle, and deer feeding on a variety of diets. Denaturing gradient gel electrophoresis fingerprinting of the internal transcr...

Brugia malayi is one of the parasitic worms which causes lymphatic filariasis in humans. Its geographical distribution includes a large part of Asia. Despite its wide distribution, very little is known about the genetic variation and molecular epidemiology of this species. In this study, the internal transcribed spacer 1 (ITS1) nucleotide sequences of B. malayi from microfilaria-positive human ...

We introduce an open source software utility to extract the highly variable ITS1 and ITS2 subregions from fungal nuclear ITS sequences, the region of choice for environmental sampling and molecular identification of fungi. Inclusion of parts of the neighbouring, very conserved, ribosomal genes in the sequence identification process regularly leads to distorted results. The utility is available ...

Filarial nematode parasites are a serious cause of morbidity in humans and animals. Identification of filarial infection using traditional morphologic criteria can be difficult and lead to misdiagnosis. We report on a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)-based method to detect and differentiate a broad range of filarial species in a single PCR. The first...

Small liver and minute intestinal flukes are highly prevalent in Southeast Asia, and in mixed infections, their eggs are difficult to differentiate morphologically in fecal samples. PCR assays targeting the ITS regions in ribosomal DNA were designed to identify and differentiate species. The PCR amplicons of Opisthorchis viverrini, Clonorchis sinensis, Haplorchis pumilio, and Haplorchis taichui...