L-methioninase has received much attention for its tumor selective anticancer activity. This study investigates the production and optimization of extracellular L-methioninase enzyme using several agro-industrial residues by Aspergillus flavipes MTCC 6337 using SSF (solid state fermentation). Effect of process variables namely substrates, incubation period, temperature, moisture content, inocul...

A longstanding suggestion posits that parents prefer to match nest volume and clutch size (clutch volume), but few studies have tested this in colonial seabirds that nest in the open. Here, we demonstrate the effects of nest-clutch volume matching on egg survival, hatching, and fledgling success in black-tailed gulls Larus crassirostris on Hongdo Island, Korea. We show that the volume mismatch,...

In mammals, males and females differ both genetically and hormonally, making it difficult to assess the relative contributions of genetic constitution and fetal environment in the process of sexual differentiation. Many reptiles lack sex chromosomes, relying instead on the temperature of incubation to determine sex. In the leopard gecko (Eublepharis macularius), an incubation temperature of 26 ...

Abstract Processes regulating the rate of oxygen depletion determine whether hypoxia occurs and extent to which greenhouse gases accumulate in seasonally ice‐covered lakes. Here, we investigate budget four arctic lakes using high‐frequency data during two winters three shallow (9–13 m maximal depth) 24 deep main basin Toolik Lake. Incubation experiments measured sediment metabolism. Volume‐aver...

The changes in water and electrolyte content of slices of Morris hepatoma 3924A induced by various conditions of incubation have been compared with the ultrastructural appearance of the tissue. Incubation at 1 degrees led to an increase of water, Na+, and Cl- content and to a loss of K+. There was simultaneous increase in size of the cells and intercellular spaces, loss of junctional complexes,...

the potential risk of metal accumulation in periurban agricultural areas is a matter for concern.the climate characteristics and carbonate content of calcareous agricultural mediterranean soils typical inthese areas favour metal accumulation at the surface level; however there is also a risk of potential metalmobility. our study focuses on the soil properties affecting metal mobility in these s...

The minnow (Phoxinus phoxinus) was raised up to the stage of swim bladder inflation at temperatures between 10 degrees C and 25 degrees C, and the time of development significantly decreased at higher temperatures. Accordingly, initiation of cardiac activity was observed at day 2 in 25 degrees C animals and at day 4 in 12.5 degrees C animals. Only a minor increase in body mass was observed duri...

The production of ethanol by Zymomonas mobilis NRRL B-4286 was studied in fed-batch cultures. Initial percent (w/v) glucose, rate of feed, and quantity of 50%; (w/v) glucose feed were varied. Glucose inhibition of growth rate occurred at concentrations greater than 8% (w/) Feed was begun after 4 h incubation. Feed volume was ca. 36%; of starting batch volume to get ca. 10%; (w/v) ethanol at har...

A direct procedure for the determination of total polysaccharides (TPS) in Camellia sinensis was set up based on the modified phenol-sulfuric acid method. The monosaccharide composition of TPS was analyzed by GC. Based on the results of GC, model monosaccharide mixtures were made which provided an adequate standard for this procedure. Through single-factor and orthogonal (L93) experiments, the ...