The susceptibility of the primary chick embryo chorioallontoic membrane cells to infectious bronchitis virus was evaluated after twenty consecutive passages in chick embryo chorioallontoic membrane cells. Virus replication was monitored by cytopathic observation, indirect immunoperoxidase, and reverse transcription polymerase chain reaction (RT-PCR). At 72 hours post-infection (p.i.) in third p...

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...

To date, multiple serotypes and genotypes of infectious bronchitis virus (IBV) have been isolated and identified. In order to provide more information on the viral evolution of IBVs, a new virulent strain named GX-NN09032, isolated from Guangxi, China, in 2009, was sequenced, and phylogenetic and recombination analyses were conducted. Furthermore, potential recombination events associated with ...

Avian infectious bronchitis virus (IBV) defective RNAs (D-RNAs) have been used for the expression of heterologous genes in a helper-virus-dependent expression system. The heterologous genes were expressed under the control of an IBV transcription-associated sequence (TAS) derived from gene 5 of IBV Beaudette. However, coronavirus D-RNA expression vectors display an inherent instability followin...

Proventriculitis was studied by experimentally reproducing the disease in broiler chickens. One-day-old infectious bursal disease virus (IBDV) and infectious bronchitis virus (IBV) antibody positive commercial broilers and 1-day-old antibody negative specific-pathogen-free (SPF) broilers were orally gavaged with proventricular homogenates produced from the proventriculi of broilers with provent...

Abstract Avian infectious bronchitis (IB) is a highly contagious disease caused by virus (IBV), coronavirus of domestic fowl. IB major concern in the poultry industry, causing worldwide economic losses through decreased egg production and quality increasing chicken's susceptibility for secondary bacterial infections, particularly Escherichia coli . In this study, anti‐IBV activity cathelicidins...

avian infectious bronchitis (ib) is a widely distributed poultry disease that has huge economic impact on poultry industry. the continuous emergence of new ibv genotypes and lack of cross protection among different ibv genotypes have been an important challenge. the present study was done to evaluate protection caused by two different serotypes’ vaccines (massachusetts & 793/b) in order to eval...

background and aims: infectious bronchitis virus (ibv) is the etiological agent of infectious bronchitis (ib), which is an acute and highly contagious disease of the respiratory and sometimes the urogenital tracts of chickens. in iran, the disease has been identified in chicken flocks with serological and virus isolation methods. gene 3c (e) encode envelop (small membrane) protein. for envelope...

Newcastle disease virus (NDV), avian influenza virus (AIV), and infectious bronchitis virus (IBV) are the most prevalent viral pathogens in the Iranian poultry industry. This study aimed to reveal the presence of these viruses in the backyard chickens in Ahvaz, located in the Southwest of Iran. A total of 100 chickens with respiratory signs and mortality were examined by taking the blood sample...

Background and Aim: QX-like infectious bronchitis virus (IBV) is a highly avian coronavirus that causes respiratory kidney disease. It linked to increased mortality loss of performance in infected chickens worldwide, including Thailand. Thus, simple rapid diagnostic method for the diagnosis IBV needed. This study aimed develop single-step multiplex reverse transcription-polymerase chain reactio...