The U,S. Army Construction Engineering Research Laboratories (CERL) has been investigating the development of a test methodology for evaluating the service life of roofing membrane materials. As pat of this effort, CERL conducted a study to investigate the applicability of strain energy (a mechanical test) and Fourier transform infrared (FTIR) spectroscopy (a chemicat analysis) as methods for c...

Fourier transform infrared (FTIR) and step-scan time-resolved FTIR difference spectra are reported for the [carbonmonoxy]cytochrome caa(3) from Thermus thermophilus. A major C-O mode of heme a(3) at 1958 cm(-1) and two minor modes at 1967 and 1975 cm(-1) (7:1:1) have been identified at room temperature and remained unchanged in H(2)O/D(2)O exchange. The observed C-O frequencies are 10 cm(-1) hi...

Fourier transform infrared (FTIR) spectroscopy was used to study protein-cofactor interactions in photosystem II. The FTIR spectrum of each cofactor was measured as a difference spectrum upon its light-induced reaction. The spectrum included the bands of the protein moiety coupled to the cofactor, which were identified using selective isotopic labeling of amino acids. Analysis of these FTIR ban...

Atmospheric particles collected during the ICARTT 2004 field experiment at ground based sites at Appledore Island (AI), New Hampshire, Chebogue Point (CP), Nova Scotia, and aboard the R/V Ronald Brown (RB) were analyzed using Fourier Transform Infrared (FTIR) spectroscopy to quantify organic mass and organic functional groups. Several of these spectra ∗To whom correspondence should be addressed...

The versatility of FTIR spectrometry was explored by considering a variety of samples drawn from industrial applications, materials science and biomedical research. These samples included polymeric insulators, bauxite ore, clay, human hair and human skin. A range of sampling techniques suitable for these samples is discussed, in particular FTIR microscopy, FTIR emission spectroscopy, attenuated...

Time-resolved Fourier transform infrared (FTIR) difference spectra of the halorhodopsin (hR) photocycle have been collected from 3 micros to 100 ms in saturating concentrations of KCl or KBr. Kinetic analysis of these data revealed two decay processes, with time constants of tau(1) approximately 150 micros and tau(2) approximately 16 ms in the presence of either halide, with tau(2) describing t...

Legislation enacted worldwide to regulate the presence of genetically modified organisms (GMOs) in crops, foods and ingredients, necessitated the development of reliable and sensitive methods for GMO detection. In this article, protein- and DNA-based methods employing western blots, enzyme-linked immunosorbant assay, lateral flow strips, Southern blots, qualitative-, quantitative-, real-time- a...

BACKGROUND The conventional method of measuring total body water by the deuterium isotope dilution method uses gas isotope ratio mass spectrometry (IRMS), which is both expensive and time-consuming. We investigated an alternative method, using Fourier transform infrared spectrophotometry (FTIR), which uses less expensive instrumentation and requires little sample preparation. METHOD Total bod...

The effect of dehydration on the coordination and speciation of sulfate at the Fe-(hydr)oxide-H2O interface was investigated using molecular orbital/density functional theory (MO/DFT) and Fourier transform infrared (FTIR) spectroscopy. IR frequency calculations were performed at the UB3LYP/6-31+G(d) level of theory for potential sulfate (bidentate bridging, monodentate, and H-bonded) and bisulf...