is based on Master Project [4] and article [3] by Soren Klim and Stig Mortensen Objectives: Insulin and C-peptide are secreted in equi-molar amounts from the beta-cells then passing through the liver before entering systemic circulation. The variable first pass effect on insulin due to hepatic extraction makes the insulin observations unusable for determining the secretion rate. C-peptid passes...

Insulin secretion from pancreatic β-cells plays an essential role in blood glucose homeostasis and type 2 diabetes. Many genes are involved in the secretion of insulin and most of these genes can be targeted by microRNAs (miRNAs). However, the role of miRNAs in insulin secretion and type 2 diabetes has not been exhaustively studied. The expression miR-184, a miRNA enriched in pancreatic islets,...

In vitro studies of human pancreatic islets are critical for understanding normal insulin secretion and its perturbations in diabetic β-cells, but the influence of islet preparation characteristics and organ donor attributes in such experiments is poorly documented. Preparations from normal donors were tested with a standardized protocol evaluating dynamic insulin secretion induced by glucose, ...

Insulin is secreted from the -cells of the pancreatic islets by Ca 2 -dependent exocytosis of large dense core vesicles (LDCVs) (Ämmälä et al., 1993), a process that is triggered by glucose-induced electrical activity (Henquin and Meissner, 1984). In response to a step elevation of glucose, insulin secretion follows a characteristic biphasic time course (Curry et al., 1968): an initial transien...

Insulin sensitivity and insulin secretion are mutually related such that insulin resistance is compensated by increased insulin secretion. A correct judgement of insulin secretion therefore requires validation in relation to the insulin sensitivity in the same subject. Mathematical analyses of the relationship between insulin sensitivity and insulin secretion has revealed a hyperbolic function,...

Aim: To assess insulin secretion/insulin resistance index in predicting the risk for future T2DM Experimental Design and Methods: 1551 non-diabetic subjects from the San Antonio Heart Study received an OGTT with measurement of plasma glucose and insulin concentrations at 0, 30, 60, and 120 minutes at baseline and after 7-8 years of follow-up. Insulin secretion/insulin resistance index was calcu...

Impaired insulin secretion in type 2 diabetes is characterized by decreased first-phase insulin secretion, an increased proinsulin-to-insulin molar ratio in plasma, abnormal pulsatile insulin release, and heightened disorderliness of insulin concentration profiles. In the present study, we tested the hypothesis that these abnormalities are at least partly reversed by a period of overnight suspe...

Glucose tolerance declines with maturation and aging in several species, but the time of onset and extent of changes in insulin sensitivity and insulin secretion and their contribution to changes in glucose tolerance are unclear. We therefore determined the effect of maturation on glucose tolerance, insulin secretion, and insulin sensitivity in a longitudinal study of male and female sheep from...

Objective(s): Ghrelin is a peptide hormone that has been shown to have numerous central and peripheral effects. The central effects including GH secretion, food intake, and energy homeostasis are partly mediated by Kiss1- KissR signaling pathway. Ghrelin and its receptor are also expressed in the pancreatic islets. Ghrelin is one of the key metabolic factors controlling insulin secretion from t...

Acute insulin secretion determines the efficiency of glucose clearance. Moreover, impaired acute insulin release is characteristic of reduced glucose control in the prediabetic state. Incretin hormones, which increase β-cell cAMP, restore acute-phase insulin secretion and improve glucose control. To determine the physiological role of the cAMP-dependent protein kinase (PKA), a mouse model was d...