Among 80 types of yeast isolated from wild flowers in Daejeon, Korea, two species that have not yet been identified by phylogenetic analysis of the internal transcribed spacer-2 (ITS2) genes and 26S rDNA sequences were identified as Candida sp. 44-C-1 and Cryptococcus sp. 9-D-1. Neither of the newly identified species formed ascospores, while Candida sp. 44-C-1 formed pseudomycelium and Cryptoc...

The location of the 5.88 rDNA within the internal transcribed spacer has been found by restriction and sequence analysis. These analyses indicate the deletion of a dinucleotide from the known rRNA sequence. Regions to the 5' and 3' of the gene contain both uncommon sequences and palindromic structures which might provide potential control points. A secondary structure model is suggested for the...

30 milled rice samples were collected from retailers in four states of Malaysia. These samples were evaluated for Eurotium spp. contaminations by direct plating on malt extract salt agar (MESA). All Eurotium were isolated and identified based on morphology and nucleotide sequences of internal transcribed spacer 1 (ITS1) and ITS2 of the rDNA.  Four Eurotium species (E. rubrum, E. amstelodami, E....

Plant parasitic nematode genera commonly found associated with sugarcane were collected from trial sites in various regions of the sugar industry. Nucleic acids were extracted and the rDNA internal transcribed spacer 1 (ITS1) region amplified. Identification of the important genera was possible by size differentiation of the amplification products. Sequencing of the ITS1 region was used to conf...

While surveying the diversity of fungi of the order Mucorales, two isolates, EML-PUKI12-1 and EML-PUKI06-1, were obtained from the gut of soldier fly larvae inhabiting the bulrush at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. The isolates were confirmed as Mucor irregularis and Mucor fragilis species, respectively, based on the morphological characteristics and...

A seed-borne fungus, Curvularia sp. EML-KWD01, was isolated from an indigenous wheat seed by standard blotter method. This fungus was characterized based on the morphological characteristics and molecular phylogenetic analysis. Phylogenetic status of the fungus was determined using sequences of three loci: rDNA internal transcribed spacer, large ribosomal subunit, and glyceraldehyde 3-phosphate...

Abstract Species of Trichogramma Westwood, 1833 (Hymenoptera: Trichogrammtidae) are frequently used as biological control agents against Lepidoptera, but practical application these egg endoparasitoids complicated because their complex taxonomy. This study aimed to compare sequences internal transcribed spacer regions ribosomal DNA (ITS2-rDNA) accessions with those deposited in GenBank order ac...

Sooty blotch and flyspeck occur on fruit surfaces and result in economic losses due to less attractive appearance. Sooty blotch fungi form dark mycelial mats whereas flyspeck fungal agents are well characterized with black, sclerotium-like bodies on fruit surface. Whilst more than 60 species have been reported in association with these two fungal diseases, thus far they have not been studied in...