Glass microelectrodes filled with a saturated solution of methyl blue or fast green in 1.0M potassium acetate can be used to mark penetrated ne. urons from which intracellular recordings have been made. Many cells can be marked in one experiment and can easily be located in subsequent histologic section.

The patch clamp is a fundamental tool for neuroscientists, offering insights that have shaped our understanding of the brain. Advances in nanotechnology suggest that the next generation of recording methods is now within reach. We discuss the complexity and future promise of applying nanoscience to neural recording.

Micro-machined silicon needles capable of penetrating through cell membranes were fabricated and tested for intracellular sensing applications. The fabricated needles have sharp tips (diameter <1 μm), they are long (>300 μm) and exhibit high mechanical strength. The needles were tested for extraand intra-cellular neuronal recording applications. To prepare the needles for neuronal recording, th...

1. Since the inception of the patch-clamp technique, single-channel recording has made an enormous impact on our understanding of ion channel function and its role in membrane transport and cell physiology. 2. However, the impact of single-channel recording methods on our understanding of intracellular Ca2+ regulation by internal stores is not as broadly recognized. There are several possible r...

Electrode technology for electrophysiology has a long history of innovation, with some decisive steps including the development of the voltage-clamp measurement technique by Hodgkin and Huxley in the 1940s and the invention of the patch clamp electrode by Neher and Sakmann in the 1970s. The high-precision intracellular recording enabled by the patch clamp electrode has since been a gold standar...

Semiconductor nanowires configured as the active channels of field-effect transistors (FETs) have been used as detectors for high-resolution electrical recording from single live cells, cell networks, tissues and organs. Extracellular measurements with substrate supported silicon nanowire (SiNW) FETs, which have projected active areas orders of magnitude smaller than conventional microfabricate...

silver nanoparticles are increasingly used in various fields of biotechnology and applications in the medicine. objectives of this study were optimization of production of silver nanoparticles using biotransformations by fusarium oxysporum, and a further study on the location of nanoparticles synthesis in this microorganism. the reaction mixture contained the following ingredients (final concen...