Phosphonomycin, (-)-cis-l, 2-epoxypropylphosphonic acid, was isolated from fermentation sources as a crystalline calcium salt. Methods for ionexchange adsorption, ion-exchange chromatography using both anionand cation-exchange resins, gel-permeation chromatography, adsorption chromatography and solvent partition were investigated. The overall purification was about 5,000 fold with a yield of 12...

prostate-specific antigen (psa) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a cm-sephadex c-50 and by gel filtration on a sephacryl s-200 column. a single 33-kda protein band appeared in sds-page. high pressure liquid chromatography (hplc) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...

calystegines were isolated from the root cultures of physalis divaricata, p. pubescens, p. philadelphia,and p. philadelphia (solanaceae). calystegines of p. divaricata were identified as calystegine a3, a5, b1 and b2, with concentrations of 6.99, 4.41, 8.52, and 14.7 ?g/g in fresh mass, respectively. root cultures of p.pubescens contain calystegine a3, b1 and b2, p. philadelphia only contains c...