Considerable enzymological diversity underlies the capacity for biosynthesis of aromatic amino acids in nature. For this biochemical pathway, higher plants as a group exhibit a uniform pattern of pathway steps, compartmentation, and catalytic, physical and allosteric properties of enzymes. This biochemical pattern of higher plants contains a collection of features which are completely different...

THE recent interest in organ-specific patterns of lactate dehydrogenase (LDH) isoenzymes (WIELAND and PFLEIDERER, 1957; WROBLEWSKI, Ross and GREGORY, 1960; PLAGEMANN, GREGORY and WROBLEWSKI, 1960; WROBLEWSKI and GREGORY, 1961) stimulated search for isoenzyme pattern differences among regions of the brain. VAN DER HELM (1962) found no isoenzymic differences among several regions of the human bra...

We investigated 28 cases of pediatric malignancy in which total lactate dehydrogenase (LD, EC 1.1.1.27) activities were increased and isoenzyme LD-1 exceeded LD-2 (flipped pattern). Of these, 11 had a flipped pattern at presentation and 17 showed a flipped pattern during chemotherapy. Those with flipped patterns at presentation were four with germ-cell tumors, one with acute lymphocytic leukemi...

The activity and presence of isoforms of NADP-dependent malic enzyme (NADP-ME, EC 1.1.1.40) were studied in non-transgenic and transgenic Nicotiana benthamiana plants containing potyviral gene for helper component protease (HC-pro) and in plants infected by Potato virus Y strain NTN (PVY(NTN)). No significant changes in enzyme activities and isoenzyme pattern were observed due to foreign gene i...

background: leishmaniasis is endemic in iran. different species of leishmania (l.) parasites are causative agents of this disease. correct identification of leishmania species is important for clinical studies, prevention, and control of the diseases. mix up of leishmania isolates is possible in the laboratory, so there is need for verification of species for isolates of uncertain identity. dif...

We assayed the isoenzymes of lactate dehydrogenase (EC 1.1.1.27) in commercial quality control sea and several animal tissue extracts, using electrophoresis. We compared the Km values and activation energies of the isoenzymes, in order to find suitable animal tissue sources with a similar isoenzyme profile to that of human serum lactate dehydrogenase. Some of the control sera contained only one...

during the period from 1996 to 1997, 45 isolates of leishmania were recovered frompatients suspected of cutaneous leishmaniasis (cl) and one from a gerbil. these specimens werereceived from various parts of iran. isoenzyme profiles of these isolates were compared with those ofreference strains of l.tropica, l.major and l.infantum using cellulose acetate electrophoresis (cae) and10 enzyme system...

For a number of lysosomal hydrolases, abnormally expressed forms have been found to occur in childhood and adult leukemia. Most of these abnormalities are confined to the physicochemical properties of the enzymes, e.g., abnormal electrophoretic mobility, change of isoelectric point, and alteration of isoenzyme pattern. All this has been described for the hexosaminidase system in human leukemia ...

Abnormal electrophoretic pattern of serum lactate dehydrogenase isoenzyme is shown. In this case electrophoretic pattern showed a sharp single band between lactate dehydrogenase 4 and lactate dehydrogenase 5 and broad band between lactate dehydrogenase 2 and lactate dehydrogenase 4. This abnormality was suggested to be caused by the presence of lactate dehydrogenase and immunoglobulin G (kappa ...