BACKGROUND Ovarian tissue cryopreservation followed by transplantation after cancer remission is the most commonly applied fertility restoration approach in very young girls and women who require immediate cancer therapy. However, clinicians strongly advise against reimplantation of one's own ovarian tissue when there is a high risk of recurrence after grafting. For these patients, development ...

The aim of the present study is to understand the hormonal mechanisms of the effect of malnutrition on ovarian follicle functions. For this purpose, we examined the effect of malnutrition/serum deprivation, addition of metabolic hormones and gonadotropin (IGF-I, leptin and FSH) and their combination on the release of progesterone (P4), testosterone (T), estradiol (E2) and insulin-like growth fa...

OBJECTIVE Improvements in cancer treatment have allowed more young women to survive. However, many cancer patients suffer from ovarian failure. Cryopreservation is one of the solutions for fertility restoration in these patients. The cryopreservation of isolated follicles is a more attractive approach in the long term. Many endocrine and paracrine factors can stimulate the granulosa cells of pr...

The mechanisms that regulate the gradual exit of ovarian follicles from the non-growing, primordial pool are very poorly understood. A better understanding of the signals that initiate follicular growth in mammals, and of the conditions necessary for sustained growth of early preantral follicles in vitro, could have practical implications for contraception, alleviation of infertility, and regul...

Objective In the present study, the effects of alginate (ALG) concentration and ovarian cells (OCs) on the development and function of follicles were simultaneously evaluated. MaterialsAndMethods In the first step, preantral follicles (100-130 µm in diameter) were mechanically isolated from the ovaries of 2-week-old NMRI mice, encapsulated in the absence or presence of OCs in 0.5, 0.75 and 1% A...

Organ regeneration is becoming a promising choice for many patients; however, many details about the mechanisms underlying organ regeneration remain unknown. As regenerative organs, hair follicles offer a good model to study the mechanisms associated with regenerative medicine. The relevant studies have mainly focused on donor cells, and there are no systematic studies involving the effect of h...

Background: The aim of this study was to investigate the compare of Conventional (CV) and Direct Cover (DCV) vitrification and effect of sodium selenite(SS) that supplemented with FBS and BSA on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and glutathione peroxide (GPx) activity of cultured pre-antral follicles derived from vitrified and non-vitrified ovarian tissu...

Background: The majority of thousands of oocytes in the ovaries are small, non-growing and reside in preantral follicles (PFs). The development of a culture system for preantral follicle may be very useful for understanding the complex mechanism in folliculogenesis at early stages of development as wells as could offer a significant way for the propagation of livestock, including goats. This pa...

Basic fibroblast growth factor (bFGF) is involved in cell proliferation, differentiation, and angiogenesis. It has long been known that bFGF acts as a powerful mitogen for various mammalian granulosa cells in culture. To investigate the possible involvement of bFGF expression in follicle initiation and growth in vivo, we performed nested RT-PCR on ovarian cortical biopsies and quantitative PCR ...

objective: in vitro ovarian follicle culture provides a tool to investigate folliculogenesis and may be used as an assisted reproductive technology )art(. this study aims to compare survival and development rates in mouse ovarian follicles after two and three dimensional in vitro cultures. methods: preantral follicles were isolated from the ovaries of 14-day old female mice and cultured in α-m...