(b) Sedimentation equilibrium: an experiment performed at a lower speed so that the sedimentation and backdiffirsion forces are of comparable magnitudes and therefore give rise to an equilibrium distribution of solute concentration. Because there is no net transport at equilibrium, shape effects do not come into play and the distribution becomes an absolute function of molecular mass for a sing...

Rat kidney contains two distinct glutaminase isoenzymes; one of which is phosphate-dependent, the other is phosphateindependent but is strongly activated by maleate. The phosphate-dependent glutaminase is contained within mitochondria. Its activity cofractionates with mitochondrial marker activities during differential centrifugation and on sucrose gradients using both isopycnic and sedimentati...

Analytical Ultracentrifugation (AUC) is a fractionating technique based on the synchronized optical detection of the concentration profiles of the sample under a centrifugal acceleration of up to 300,000 g. The skilled choice of solvent, rotational speed and detection optics enables a variety of complementary AUC experiments due to the controlled interplay of centrifugal force, hydrodynamic fri...

Complexes of mouse main band DNA with a fraction of non-histone proteins (NHP), having a high affinity for DNA, in the absence or presence of histones have been investigated by gradient centrifugation in metrizamide. Two types of complexes were formed at an input ratio of NHP to DNA between 1 and 2.5. In metrizamide gradients a majority of DNA was found in the light complex (at the density of 1...