We have prepared a novel kind of building block (CD-PI-CD) of polyether imide (PI) with beta-cyclodextrin (beta-CD) at the both ends that self-assembles into vesicles in water. The vesicles are "reactive" in supramolecular chemistry because the host groups of beta-CD equally distribute on the inside and outside surfaces of the vesicles. Isothermal titration calorimetry (ITC) and static light sc...

The expression or replication of genes is affected by the binding of small molecule ligands and proteins to nucleic acid sequences. Such binding events are critical for the physiological integrity of organisms and therefore are of fundamental interest to life scientists. Recently, the thermodynamics driving these interactions have also become important to pharmaceutical scientists investigating...

The histamine H₂-receptor antagonists cimetidine, famotidine and nizatidine are individually encapsulated by macrocyclic cucurbit[7]uril (CB[7]), with binding affinities of 6.57 (±0.19) × 10³ M(-1), 1.30 (±0.27) × 10⁴ M(-1) and 1.05 (±0.33) × 10⁵ M(-1), respectively. These 1:1 host-guest inclusion complexes have been experimentally examined by ¹H-NMR, UV-visible spectroscopic titrations (includ...

In this work, isothermal titration calorimetry (ITC) combined with zeta potential measurements was used to study the binding and partitioning of three beta-blockers, alprenolol, labetalol and propranolol, and the local anaesthetic tetracaine into liposomes. The thermodynamic parameters of enthalpy, entropy, the Gibbs energy and the binding constant were determined using the one site model. Furt...

Native alpha-cyclodextrin (alpha-CD) is found to spontaneously form films at aqueous solution/air interfaces. Shape-response measurements to volume perturbations on drops hanging from a capillary indicate that temperature and sodium dodecyl sulfate (SDS) concentration strongly modify the viscoelastic properties of such films. By using isothermal titration calorimetry (ITC), Brewster angle micro...

Within the past 10 years, major advances in the design and development of differential scanning calorimeters (DSC) (1) and isothermal titration calorimeters (ITC) (2) have resulted in an unparalleled level of sensitivity, stability, and reproducibility in calorimetric measurements of large molecules. These improvements have allowed the thermal stability and ligand binding processes of biologica...

Effects of ?-cyclodextrin, ?CD, on refolding of lysozyme was investigated at pH 12 employing isothermal titration calorimetry (ITC) at 300K in 30mM Tris buffer solution. ?CD was employed as an anti-aggregation agent and the heats obtained for lysozyme+?CD interactions are reported and analyzed in terms of the extended solvation model. It was indicated that there are two sets of identical and no...

NADPH oxidase (NOX) is a multidomain protein that transfers an electron from bound by the dehydrogenase (DH) domain through transmembrane (TM) to final acceptor, O2, form superoxide. The also binds FAD cofactor. A recently discovered prokaryotic NOX homolog, SpNOX, has emerged as ideal model for study of NOXes because its robust expression and activity. An SpNOX DH domain-only construct reduces...

The binding of four bromobenzotriazoles to the catalytic subunit of human protein kinase CK2 was assessed by two complementary methods: Microscale Thermophoresis (MST) and Isothermal Titration Calorimetry (ITC). New algorithm proposed for the global analysis of MST pseudo-titration data enabled reliable determination of binding affinities for two distinct sites, a relatively strong one with the...

In an effort to determine the thermochemistry of established organometallic transformation, well documented reaction alkynes with a palladacycle was investigated by isothermal titration calorimetry (ITC). Although mechanism insertion unsaturated substrates into Pd−C bond cyclopalladated compounds is known, no information available so far about their thermochemistry. The enthalpies reactions Ph−...