Few countries in sub-Saharan Africa make systematic searches for antigens C, c, E, and e of the Rh and Kell system antigens in the donor and recipient, thereby exposing transfused patients. Purpose and Objectives. In this paper, we propose to determine the red cell Rh and Kell blood groups among blood donors from traditional techniques to improve medical care of transfused patients. This study ...

Serology, defined as antibody-based diagnostics, has been held as the analytical gold standard in transfusion medicine. However, at the present time, the influence of molecular diagnostics in transfusion medicine is rapidly growing. These methods can improve tissue typing (HLA typing), increase safety of blood products (NAT testing of infectious diseases), and enable blood group typing in diffi...

K ell is a major human blood group system that is highly polymorphic, and at present it is known to express 28 different alloantigens. After the ABO and Rh systems, Kell is the most important blood group system in transfusion medicine, as some of the antigens are potent immunogens and their antibodies can cause severe transfusion reactions in mismatched blood transfusions and fetal anemia in fe...

Two hundred blood samples obtained from volunteer blood donors at the Blood Bank, Army Institute of Pathology were studied for red cell groupings in the ABO, Rh, MNSs, Duffy, Lewis. P. Kell, Lutheran and Kidd Systems. Each sample was tested by the gel test using five cards; the ABO-Rh card, Diaclon Rh sub groups + K card, Antigen profile I card (P, Le(a), Le(b), Lu(a), Lu(b)), Antigen profile I...

A human monoclonal anti-Kell (K1) antibody secreted by an Epstein-Barr virus (EBV)-transformed B-cell line was used for binding studies and immunopurification of the K1 blood group antigen. The 125I-labeled antibody bound to 4 to 5 x 10(3) and 2.5 to 3 x 10(3) antigenic sites on K1K1 and K1K2 erythrocytes, respectively, with an affinity constant of 5 x 10(8) mol/L-1. Immunoprecipitation analysi...