The Limulus amoebocyte lysate (LAL) test has replaced about 80% of the use of the rabbit pyrogen test. Ideally, human-based in vitro tests are needed, to replace the remaining use of the rabbit test and the use of the LAL test. The progress of an EU-funded project is described, in which a number of in vitro tests, based on the human fever reaction are passing through a prevalidation study on th...

The plethora of new publications on non-alcoholic fatty liver disease (NAFLD) in both pediatric and adult populations worldwide is testimony to the scale of this problem (Abd El-Kader and El-Den Ashmawy, 2015; Sanyal et al., 2015). Projections on the numbers of cases as well as related liver transplantations in the coming decades underscore the urgent need for expanded research into the mechani...

Despite being trained in pre-service teacher education programs, most EFL teachers are underprepared when faced with language assessment-related activities. Part of the problem emanates from the fact that Language Assessment Literacy (LAL) as a construct has not been well defined by experts. The purpose of this study was to pinpoint the components of LAL in the Iranian EFL context using an adap...

Lysosomal acid lipase (LAL) is a key enzyme in lipid metabolism. Initial reports have suggested a role for a relative acquired LAL deficiency in non-alcoholic fatty liver disease (NAFLD)-however, it is still unclear whether this mechanism is specific for NAFLD. We aimed to determine LAL activity in a cohort of NAFLD subjects and in a control group of hepatitis C virus (HCV)-infected patients, i...

Non-alcoholic fatty liver disease (NAFLD) is characterized by intra-hepatic fat accumulation and mechanisms involved in its pathogenesis are not fully explained. Lysosomal Acid Lipase (LAL) is a key enzyme in lipid metabolism. We investigated its activity in patients with fatty liver. LAL activity (nmol/spot/h) was measured in 100 adult healthy subjects (HS) and in 240 NAFLD patients. A sub-ana...

Disadvantages of the regulatory pyrogen test to assure safety of the end-product Human Serum Albumin (HSA) for parenteral use call for the implementation of an alternative test. In the current study, 16 HSA batches were assayed for pyrogens in parallel with the Rabbit Pyrogen Test, conventional and endotoxin-specific LAL assay and monocyte activation test (MAT). It was found that all HSA batche...