This investigation was conducted to identify polymorphism of the leptin gene in Holstein cows, Mazandarani native cattle and buffaloes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The frequencies of A and B alleles were 0.4 and 0. 6 in Holstein cows, 0.56 and 0.44 in Mazandarani native cattle, 0.61 and 0.35 in buffaloes, respectively. The heterozygote genoty...

The present study compares the effect of various lectins; phytohaemagglutinin (PHA), Pokeweed mitogen (PWM) and Concanavalin-A (Con A) on short term lymphocyte culture and morphology of chromosome in cattle and buffaloes. For the comparative studies, the whole blood culture from 5 cattle and 5 buffaloes in triplicate were set up by adding 3 different types of lectins. Results based on physical ...

The rickettsia Anaplasma marginale is the etiologic agent of bovine anaplasmosis, an important tick-borne disease affecting cattle in tropical and subtropical regions of the world. In endemic regions, the genetic diversity of this pathogen is usually related to the high prevalence of the disease in cattle. The major surface protein 1 alpha (MSP1a) has been used as a marker to characterize the g...

A study was undertaken to assess the sensitivity and specificity of Counter Immunoelectrophoresis (CIE) in the diagnosis of hydatidosis in buffaloes using sera samples collected at the time of slaughter. One hundred sera samples from buffaloes were screened for hydatidosis using fertile buffalo hydatid cyst fluid as antigen by CIE. Out of the 100 sera samples used, 61 were positive for hydatid ...

We aimed to determine the seroprevalence of infection with Neospora caninum, Leptospira, and bovine herpesvirus type 1 and risk factors associated with these infections in water buffaloes in Veracruz State, Mexico. Through a cross-sectional study, 144 water buffaloes (Bubalus bubalis) raised in 5 ranches of Veracruz were examined for anti-N. caninum and anti-bovine herpesvirus type 1 antibodies...

Research into the application of electrocardiography in buffaloes has been lacking when compared to other species. However, identification of altered health states, whether for identification of healthy animals or for the measurement of treatment effects is vital for research. Several electrocardiogram (ECG) changes were shown to occur in buffaloes naturally infected with Theileria annulata inc...

Aim: Outbreak of bovine brucellosis in buffaloes aborted in a village Mahuan of District Mainpuri, Uttar Pradesh is described. Materials and Methods: A total of 15 serum samples were collected from a aborted buffaloes. All the sera samples were tested by serological tests, Rose Bengal Plate Test (RBPT) and Standard Tube Agglutination Test (STAT). Results: Clinical history of the condition compl...

The present study was designed to determine the quarter-wise comparative prevalence of mastitis in buffaloes and crossbred cows. Milk samples collected from 50 dairy buffaloes and 50 crossbred cows were tested for subclinical mastitis by Surf Field Mastitis Test. In addition, all milk samples were processed for isolation and identification of pathogens. In buffaloes, overall prevalence of subcl...

Fifty one dairy buffaloes in their last two months of gestation were selected at seven peri-urban commercial farms located within a radius of 70km around Peshawar. These animals were monitored from parturition until 150 days post-partum. After parturition, rectal examination of reproductive organs was carried out. Estrus detection was made through visual signs and use of intact bulls. Milk samp...

The objective of this study was to investigate the effect of carbohydrate source and cottonseed meal level in the concentrate on feed intake, nutrient digestibility, rumen fermentation and microbial protein synthesis in swamp buffaloes. Four, 4-yr old rumen fistulated swamp buffaloes were randomly assigned to receive four dietary treatments according to a 2×2 factorial arrangement in a 4×4 Lati...