A method is proposed for measuring the cytocidal effect of L-asparaginase on leukemic blood cells as a possible means of screening patients for L-asparaginase therapy. On the average, L-asparaginase was more toxic to blood lymphocytes from patients with chronic lymphocytic leukemia than to lymphocytes from normal blood. A positive sensitivity test was defined as one in which 65% of lymphocytes ...

Crystalline glutaminase-asparaginase with antitumor activity was prepared from an Achromobacferaceae soil isolate organism. This enzyme has L-glutaminase and L-asparaginase activity in a ratio of 1.2:1. The purification procedure provides an over-all yield of 40 to 60% from crude cell-free extract to homogeneous glutaminase-asparaginase and is adaptable to large scale isolation of the enzyme. G...

The enzyme Asparaginase, known as L-asparagine amidohydrolase, has anti-tumor properties and is widely used a chemotherapeutic treatment for acute lymphoblastic leukemia. Many different organisms, including plants terrestrial microbes such bacteria, fungi, algae actinomycetes, have produced this enzyme. In study, 34 isolates of the actinomycetes were collected from soil water governorates in Eg...

L-Asparaginase was added to vincristine and prednisone for induction of first remission in 815 children with acute lymphocytic or acute undifferentiated leukemia. This combination resulted in an overall remission rate of 93%. The addition of L-asparaginse to the standard induction regimen using prednisone and vincristine did not significantly increase the morbidity or mortality rate during the ...

To assess the cost-utility of pegaspargase versus L- asparaginase, both followed by Erwinase in therapy sequence, as treatment option for pediatric, adolescents and adult patients with acute lymphoblastic leukemia (ALL) Greece. A published model comprised a decision tree (DT) state-transition Markov was adapted from public payer perspective to compare sequence L-asparaginase. The DT included re...

The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated the ability to gro...

Background. L-asparaginase is effective in treating canine and feline lymphoma, however chemotherapy poses a significant financial cost to veterinary clients, limiting therapy for many pets. Single dose vials result in significant drug wastage, and drug shortages limit consistent availability for pets. Hypothesis. E. coli-derived asparaginase retains enzymatic and antineoplastic activity in can...

PATIENT Male, 51 FINAL DIAGNOSIS: Encephalopaty toxic Symptoms: Confusion • disorientation • drowsiness • fever MEDICATION L-asparaginase Clinical Procedure: - Specialty: Oncology. OBJECTIVE Unknown ethiology. BACKGROUND Novel therapies have improved survival in malignancies of lymphoid origin. This improvement, however, has been at the cost of chemotherapy-related toxicities. L-asparagin...

Aggressive nature killer cell leukemia (ANKL) is a mature NK-T cell lymphoma with worse prognosis, but optimal treatment is unclear. Therefore, we analyzed the efficacy of L-asparaginase-based regimens for ANKL patients. Twenty-one patients who received dexamethasone, methotrexate, ifosfamide, L-asparaginase, and etoposide (SMILE) or etoposide, ifosfamide, dexamethasone, and L-asparaginase (VID...

L-asparaginase is an integral component of therapy for acute lymphoblastic leukemia. However, asparaginase-related complications, including the development of hypersensitivity reactions, can limit its use in individual patients. Of considerable concern in the setting of clinical allergy is the development of neutralizing antibodies and associated asparaginase inactivity. Also problematic in the...