Hee Jin Huh, M.D.*, Yun Mi Park, M.D.*, Seungok Lee, M.D., Chunhwa Ihm, M.D., Soyeon Seo, M.D., Sang Gon Lee, M.D., Joonseok Park, M.D., and Hae-il Park, M.D. Department of Laboratory Medicine, Dongguk University Ilsan Hospital, Goyang; Siemens Healthineers, Seoul; Department of Laboratory Medicine, Incheon St. Mary’s Hospital, The Catholic University of Korea, Incheon; Department of Laboratory...

The crystallization of membrane proteins in amphiphile-rich materials such as lipidic cubic phases is an established methodology in many structural biology laboratories. The standard procedure employed with this methodology requires the generation of a highly viscous lipidic material by mixing lipid, for instance monoolein, with a solution of the detergent solubilized membrane protein. This pre...

Molecular Gastroenteroly and Hepatology column, “Paths and Places for GI Research Success,” developed in part in response to the American Gastroenterological Association’s goal of providing more targeted information and services to the diverse groups within the membership. Gastrointestinal (GI) researchers, such as the American Gastroenterological Association’s constituency, are heterogeneous. ...

From 1980 to 1990 we found progressive increases in workload (number of billable tests; 12.1% per year), staffing [number of full-time equivalents (FTEs); 5.6% per year], "revenues" (gross billings; 25.8% per year), and direct cost (12.9% per year) in the clinical chemistry laboratory of a large tertiary-care university medical center. The increase in direct cost was mainly attributable to an i...

BOTJLANGER and BISERrE (1) used a solution of acid acetone for the extraction of amino acids in biologic material. The separation of amino acids on buffered papers described by Ryb#{225}r, Touek, and Hais, (2) and Hais and Hordovs/q (3) used the combination of tertiary butanolborate buffer system (pH 8.5) for separation of amino acids on paper. In our laboratories we have developed a chromatogr...

Sarah E. Hochman,a Theresa F. Madaline,a,b Samuel C. Wassmer,c,d Emmie Mbale,e,f Namjong Choi,g Karl B. Seydel,h,i Richard O. Whitten,j Julie Varughese,a,b Georges E. R. Grau,d Steve Kamiza,k Malcolm E. Molyneux,f,l Terrie E. Taylor,h,i Sunhee Lee,g Danny A. Milner, Jr.,m,n Kami Kima,g,o Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USAa; Montefiore Medical Cente...

" Clinical judgment is always necessary, and … is superior to any test or technological advance. " Stat testing is unquestionably inherent to medicine, and the issue of stat EEG is a thorny one for every EEG laboratory. Most EEG laboratories acknowledge this as a problem, but there is no consensus on how to address it. How to deal with it is typically the responsibility of the laboratory direct...

Robert W. Redmond,*,† Irene E. Kochevar,† Marianne Krieg,‡ Glenn Smith,§ and W. Grant McGimpsey§ Wellman Laboratories of Photomedicine, HarVard Medical School, Massachusetts General Hospital, Boston, Massachusetts 02114, Pediatrics Department, MACC Fund Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, and Department of Chemistry and Biochemistry, Worcester Polytechnic ...

OBJECTIVE To evaluate the trends of ciprofloxacin resistance pattern in commonly isolated gram-negative bacteria over time in a Saudi Arabian teaching hospital. METHODS A retrospective analysis was carried out for ciprofloxacin resistance patterns of 10089 isolates of gram-negative bacteria isolated from various clinical specimens submitted to microbiology laboratories at King Fahd Hospital o...

There have been several recent incidents involving the exploding of cryopreservation vials in Trust and Medical School laboratories. This occurs when liquid nitrogen (LN ) leaks into the vials and rapidly expands when removed from the tank. In one incident in our Medical School a research worker’s hand was injured. At Edinburgh University the lid of a cryovial was projected into a student’s eye...