Several beta-lactamases, enzymes that play an important part in antibiotic resistance, have been purified by affinity chromatography on boronic acid gels. The procedure is rapid, appears to be selective for beta-lactamases, and allows a one-step purification of large amounts of enzyme from crude cell extracts. We have found the method useful for any beta-lactamase that is inhibited by boronic a...

Klebsiella species normally produce a chromosomally encoded group 2b, or molecular class A, -lactamase. In most strains of Klebsiella pneumoniae the enzyme has an isoelectric point (pI) of 7.6, identical to that of the plasmidencoded -lactamases SHV-1 and SHV-2, and is assumed to be SHV-1. This -lactamase is transposon-encoded and it has been postulated that it might have arisen as a klebsiella...

Carbapenems are frequently used for severe hospital infections caused by K. pneumoniae, especially when isolates produce extended-spectrum β-lactamases...

Genes for two group 1 beta-lactamases, SRT-1 and SST-1, were sequenced. These beta-lactamases were produced by clinical isolates of Serratia marcescens, isolates GN16694 and GN19450, respectively. The resulting enzymes were 96% identical. SRT-1 hydrolyzed oxyimino cephalosporins, but SST-1 hardly hydrolyzed them. At residue 213 in the third motif, which is conserved among group 1 beta-lactamase...

Citation: van den Akker F and Bonomo RA (2018) Exploring Additional Dimensions of Complexity in Inhibitor Design for Serine β-Lactamases: Mechanistic and Intraand Inter-molecular Chemistry Approaches. Front. Microbiol. 9:622. doi: 10.3389/fmicb.2018.00622 Exploring Additional Dimensions of Complexity in Inhibitor Design for Serine β-Lactamases: Mechanistic and Intraand Inter-molecular Chemistry...

The aim of this study was to investigate the pathogenic potential and antibiotic resistance 59 Escherichia coli isolates from ready-to-eat (RTE) street food (n = 31) drinking water 28) sold in city Maputo, Mozambique. were characterized by XbaI subtyping analysis via pulsed field gel electrophoresis. Multiplex PCRs performed targeting five virulence genes (stx, lt, st, astA, eae) three groups a...

II. The occurrence of modified penicillin-binding sites Modified PBPs have a lower affinity for β-lactam antibiotics, requiring clinically unattainable concentrations of the drug to effect its bactericidal activity Example: penicillin resistance in Streptococcus pneumoniae (pneumococcus) is caused by altered PBPs I. Production of β-lactamases This family of enzymes can inactivate penicillins by...

The occurrence of multidrug-resistant Serratia marcescens strains producing metallo-β-lactamases or extended-spectrum β-lactamases represents a serious public health threat. Here, we report the draft genome sequence of a multidrug-resistant carbapenemase-producing Serratia marcescens isolate recovered from the bronchoalveolar lavage specimen of a patient suffering from chronic obstructive pulmo...

Two isolates of Klebsiella pneumoniae possessing both TEM-1 and SHV-2 beta-lactamases were isolated from patients at the Cleveland Clinic in 1988. The beta-lactamases were discriminated and identified by using substrate hydrolysis data and an isoelectric focusing procedure in which the gel was overlaid with beta-lactamase inhibitors.

The strong selective pressure exerted by excessive use of antibiotics in the last decades has increased the acquisition of resistance genes by horizontal gene transfer. Horizontal gene transfer contributed to the diversification of microorganisms by influencing traits such as metabolic potential of a bacterial cell, antibiotic resistance, symbiosis, fitness, and adaptation. The study of biotic ...