We announce the draft genome assembly of Lactococcus garvieae strain PAQ102015-99, a recently isolated strain from an outbreak of lactococcosis at a commercial trout farm in the northwestern United States. The draft genome comprises 14 contigs totaling 2,068,357 bp with an N50 of 496,618 bp and average G+C content of 38%.

Lactococcus is an economically important starter culture bacterium extensively used in the manufacture of the both soft and hard cheeses. During its growth and storage, and throughout cheese processing, it encounters a variety of stresses including osmotic, oxidative, temperature, acid and cell envelope stress. These stressors can cause damage to DNA, proteins, lipid membranes and peptidoglycan...

100 lactic acid bacterial strains isolated from traditional fermented foods (yoghurt, milk cream, sour dough and milk) were screened for bacteriocin production. Twenty six strains producing a nisin-like bacteriocin were selected. Most of these isolates gave only a narrow inhibitory spectrum, although one showed a broad inhibitory spectrum against the indicator strains tested, this strain was de...

Citation: Bartholomae M, Baumann T, Nickling JH, Peterhoff D, Wagner R, Budisa N and Kuipers OP (2018) Expanding the Genetic Code of Lactococcus lactis and Escherichia coli to Incorporate Non-canonical Amino Acids for Production of Modified Lantibiotics. Front. Microbiol. 9:657. doi: 10.3389/fmicb.2018.00657 Expanding the Genetic Code of Lactococcus lactis and Escherichia coli to Incorporate No...

The distribution, architecture, and conjugal capacity of nisin-sucrose elements in wild-type Lactococcus lactis strains were studied. Element architecture was analyzed with the aid of hybridizations to different probes derived from the nisin-sucrose transposon Tn5276 of L. lactis NIZO R5, including its left and right ends, the nisA gene, and IS1068 (previously designated iso-IS904), located bet...

Lactococcus lactis subsp. diacitilactis S50 produces a bacteriocin, designated bacteriocin S50, which has a narrow antibacterial spectrum. It was active only against Lactococcus species, including a nisin producer exhibiting a bactericidal effect. The activity of bacteriocin S50 was sensitive to proteases. It retained antimicrobial activity after being heated to 100 degrees C for up to 60 min a...

Reduced genome strain for threonine production http://www.microbialcellfactories.com/content/8/1/2 This paper deals with a key concept in genome engineering; specifically, genome reduction to remove unneeded functions and to enhance desirable cell phenotypes for biotechnology. Genomic engineering Lactococcus lactis http://www.nisr.or.jp/englishHP/report2007/ NISR07hashimoto.pdf Genome deletion ...

INTRODUCTION A case of Lactococcus lactis cremoris causing cholangitis is described. This Gram-positive organism is not routinely considered to be pathogenic in immunocompetent individuals. To our knowledge, this is the thirteenth report of invasive infection and the first of cholangitis to be reported in association with this organism. CASE PRESENTATION A 72-year-old patient presented with C...

Bacteria of the genus Lactococcus are commonly used in manufacturing dairy products.1This genus of bacteria is also being investigated for use in the biotechnology industry, as a delivery system for vaccines and other therapies.1 It is rarely considered pathogenic in humans, although several cases of clinically significant Lactococcus infection have been reported. In adults, these infections ha...