Lymphocytic choriomeninigitis (LCM) virus defective interfering (DI) particles form foci of protected cells in a monolayer under an agarose-containing overlay medium. Foci originate from one cell dually infected with at least 1 interference focus-forming unit and infectious virus. As a result, an interfering factor is produced and released which interacts with neighboring cells, thereby protect...

Lacosamide (LCM) is an antiepileptic drug approved as adjunctive therapy for partial-onset seizures in adults. It has a mechanism of action that differs from other antiepileptic drugs in that it selectively enhances sodium channel slow inactivation, which is in contrast to 'traditional' sodium channel blockers (e.g., carbamazepine, oxcarbazepine, lamotrigine and phenytoin) that primarily affect...

The ability to reliably analyze cellular and molecular profiles of normal or diseased tissues is frequently complicated by the inherent heterogeneous nature of tissues. Laser Capture Microdissection (LCM) is an innovative technique that allows the isolation and enrichment of pure subpopulations of cells from tissues under direct microscopic examination. Material obtained by LCM can be used for ...

Lacosamide (LCM) is a novel agent that has been developed as an antiepileptic drug. In vitro studies suggest that LCM modulates voltage-gated sodium channels by enhancing their slow inactivation. In addition, LCM seems to interact with collapsin-response mediator protein 2 and thus may mediate neuronal plasticity. LCM has an elimination half-life of 13 hours, no relevant protein binding, and do...

Laser capture microdissection (LCM) offers a reliable cell population enrichment tool and has been successfully coupled to MS analysis. Despite this, most proteomic studies employ whole tissue lysate (WTL) analysis in the discovery of disease biomarkers and in profiling analyses. Furthermore, the influence of tissue heterogeneity in WTL analysis, nor its impact in biomarker discovery studies ha...

AUTHORS Vanessa VIEIRA / CICECO AVEIRO INSTITUTE OF MATERIALS, UNIVERSITY OF AVEIRO; ASSOCIATE LABORATORY LSRE-LCM, POLYTECHNIC INSTITUTE OF BRAGANÇA; CIMO MOUNTAIN RESEARCH CENTER, POLYTECHNIC INSTITUTE OF BRAGANÇA, CICECO AVEIRO INSTITUTE OF MATERIALS, DEPARTMENT OF CHEMISTRY, CAMPUS UNIVERSITÁRIO DE SANTAGO, AVEIRO Olga FERREIRA / ASSOCIATE LABORATORY LSRE-LCM, POLYTECHNIC INSTITUTE OF BRAGA...

INTRODUCTION In recent year, the design concepts of spinal implants alternation from traditional stable fusion to restore normal spinal range of motion (ROM). Therefore, the method of the spinal implants in vitro test was advanced from traditional load control method (LCM) to displacement control method (DCM) [1-3]. In real life, it is still not clear whether to use LCM or the DCM to evaluate t...

We describe an approach to optical lithography using light-scattering contact masks with protruding elements that couple light into a photoresist. This method differs from conventional contact lithography in two important ways. First, because portions of the light-coupling mask ~LCM! are made from a polymer, intimate contact with the resist occurs over large areas without additional load. This ...

Laser capture microdissection (LCM) facilitates the isolation of individual cells from tissue sections, and when combined with RNA amplification techniques, it is an extremely powerful tool for examining genome-wide expression profiles in specific cell-types. LCM has been widely used to address various biological questions in both animal and plant systems, however, no attempt has been made so f...