AIM To assess five main histological features of gastritis in gastric mucosa colonized with Helicobacter pylori before and after the treatment. METHODS Histologic assessment of H. pylori-associated gastritis was performed according to the Sydney classification before and after the treatment in 97 patients. Two additional parameters - the presence of lymphocytic aggregates and coccoid forms of...

Fig. 1. (A) Peripheral blood shows neutrophils with toxic granules and two intra-cytoplasmic yeast forms of Histoplasma capsulatum in the cell on the left (May-Grunwald Giemsa, ×1,000). Inset: The fungus was brightly positive for periodic acid–Schiff stain (hematoxylin counterstain, ×1,000). (B) Hemodilute bone marrow smears showed dysgranulopoiesis with 5% blasts (May-Grunwald Giemsa, ×1,000)....

For enumeration of intracellular organisms (ICO) in bronchoalveolar lavage fluid samples, the May-Grünwald-Giemsa (MGG) stain displayed higher interobserver agreement than the acridine orange and Gram stains. The MGG stain offered a reliable enumeration of ICO when 200 cells were counted by one observer.

Giemsa-stained blood smears are mainly used for microscopic examination to diagnose malaria. However, they may also be subjected to polymerase chain reaction (PCR) to confirm diagnosis or for retrospective studies requiring the analysis of old smears. We investigated the possibility of DNA contamination occurring during automated Giemsa staining or due to the failure to clean the oil-immersion ...

We compared a nested PCR assay and microscopic examination of Giemsa-stained blood films for detection and identification of Plasmodium spp. in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal.

CAPTAIK, I.M.S. Combined Indian Military Hospital, Sang or, C. P. Requirements 1. Distilled water. 2. The usual Leishman stain?0.5 per cent in methyl alcohol. It should be made up in sufficient amount to last for a week. No other alcoholic solution should be substituted for methyl alcohol. Method of preparing and staining the film 1. Take a fairly big drop of blood and spread to the size of a s...

The present study was aimed to assess the incidence of T. evansi infections in camel Saurashtra region Gujarat, India, through microscopy and polymerase chain reaction (PCR) methods. Blood samples were collected from 192 camels, randomly. diagnostic sensitivity specificity Field’s stain, Giemsa stain RoTat1.2 assays for detection blood evaluated. infection recorded camels based on PCR 3.23%, 8....

Aim: Helicobacter pylori infection has been ascertained to play pivotal role in the pathogenesis of chronic gastritis and gastric neoplasia.1The present study was performed evaluate diagnostic accuracy H&E stain Giemsa for histological diagnosis helicobacter by taking immunohistochemical staining as a gold standard. Methods: A total 155 cases were included our study. The received biopsies f...

AIMS To evaluate three tests used routinely for the diagnosis of herpes simplex virus (HSV) keratitis. METHODS Corneal scrapings from 28 patients with clinically typical dendritic corneal ulcer suggestive of HSV keratitis, and 30 patients with clinically non-viral corneal ulcers, were tested by (i) Giemsa stain for multinucleated giant cells, (ii) immunofluorescence assay (IFA) for HSV-1 anti...