abstract          background and objective: cutaneous leishmaniasis is a parasitic disease and a health problem in different parts of iran, especially two cities of mashhad and chabahar. due to morphological similarities of most leishmania species and difference in reservoirs of l. major and l. tropica, it is necessary to determine the parasite specie to combat the disease. thus, this study use...

Cutaneous leishmaniasis is caused by different species of the Leishmania genus. Leishmania(Leishmania) infantum, causing cutaneous leishmaniasis, has been described in patients living in areas where visceral leishmaniasis is endemic. In this study, it was possible to characterize this species in seven slides from cutaneous tissue imprints from patients with cutaneous leishmaniasis in the State ...

Brazil is one of the most important endemic areas for leishmaniasis worldwide. Protected areas that are tourist attractions likely present an important risk of transmission of cutaneous leishmaniasis (CL). Furthermore, with the geographical expansion of visceral leishmaniasis (VL), several studies have recorded the occurrence of its vector, Lutzomyia longipalpis, and cases of human and canine V...

In this study, PCR assays targeting different Leishmania heat-shock protein 70 gene (hsp70) regions, producing fragments ranging in size from 230-390 bp were developed and evaluated to determine their potential as a tool for the specific molecular diagnosis of cutaneous leishmaniasis (CL). A total of 70 Leishmania strains were analysed, including seven reference strains (RS) and 63 previously t...

Leishmaniasis is a complex parasitic disease from a taxonomic, clinical and epidemiological point of view. The role of genetic exchanges has been questioned for over twenty years and their recent experimental demonstration along with the identification of interspecific hybrids in natura has revived this debate. After arguing that genetic exchanges were exceptional and did not contribute to Leis...

The detection and identification of natural infections in sand flies by Leishmania protozoan species in endemic areas is a key factor in assessing the risk of leishmaniasis and in designing prevention and control measures for this infectious disease. In this study, we analyzed the Leishmania DNA using nuclear ribosomal internal transcript spacer (ITS) sequences. Parasite DNA was extracted from ...

Article history: Received: .................. Accepted: ................... Available online: ........ ZJRMS 2014; X(X): XX-XX Background: Genotyping of Crithidia species is critical in order to design appropriate programs for control and prevention of cutaneous leishmaniasis (CL). Materials and Methods: In this descriptive-analytical study, 602 samples were isolated from suspicious patients wi...

Objective: To identify the Leishmania species being responsible for cutaneous leishmaniasis in Multan. Method: Parasites were isolated from clinically and parasitologically confirmed lesions of cutaneous leishmaniasis from 30 patients by fine needle aspiration (FNA). The bioptical materials were then cultured in Evans Tobie's medium and parasites isolated were identified by isoenzyme electropho...