Cooking methods affect the compositions of Lentinus edodes metabolites. Nevertheless, little information is available on specific impact different cooking via metabolomic analysis. This study determined influence boiling, steaming, air-frying, and roasting profiles based UHPLC-Q-Exactive Orbitrap MS/MS in combination with chemometrics. A total 990 metabolites were detected classified into 11 su...

Shiitake mushrooms (Lentinula edodes) containing β-glucans may be beneficial for human health; they have been used in the treatment of cancer, hypertension, and high cholesterol levels. The objective of this study was to determine the β-glucan content in different sections of the fruiting bodies and mycelia of ten shiitake mushroom cultivars. The measured β-glucan content ranged from 20.06 ± 1....

Lentinula edodes, one of the most popular, edible mushroom species with a high content of proteins and polysaccharides as well as unique aroma, is widely cultivated in many Asian countries, especially in China, Japan and Korea. As a white rot fungus with lignocellulose degradation ability, L. edodes has the potential for application in the utilization of agriculture straw resources. Here, we re...

In mating of Lentinula edodes, dikaryotic strains generated from certain monokaryotic strains such as the B2 used in this study tend to show better quality of fruiting bodies regardless of the mated monokaryotic strains. Unlike B2, dikaryotic strains generated from B16 generally show low yields, with deformed or underdeveloped fruiting bodies. This indicates that the two nuclei in the cytoplasm...

28, 36, and 41 kDa, On the other hand, Kurosawa et al.4) found an alkaline RNase in addition to three acid nucleases in frcsh fruit bodies of L, edodes confirming the results of Endo et aLi) To establish more precise information on these enzymes, we tried to isolate and characterize each nuclease or RNase in L. edodes. First, we isolated an acid RNase, RNase Le2, and established the prirnary st...

In the course of our screening for antithrombotic compounds using platelet rich plasma from bovine slaughter blood, 2-methoxy-5-methyl-1,4-benzoquinone (1) has been isolated from mycelial cultures of Lentinus adhaerens. The compound inhibits the U46619-induced aggregation of human blood platelets with an IC50 of 2.5 micrograms/ml (16.45 microM) and is a new thromboxane A2 receptor antagonist. T...

A pressurized water extraction (PWE) method was developed in order to extract β-glucans with bile acids-binding capacities from cultivated mushrooms (Agaricus bisporus, Lentinula edodes, and Pleurotus ostreatus) to be used as supplements to design novel foods with hypocholesterolemic properties. Extraction yields were higher in individual than sequential extractions being the optimal extraction...

Lentinula edodes, one of the most important edible mushrooms in China, is affected heavily by the infection of green mold that overgrows mushroom mycelia. We collected the diseased samples from main L. edodes cultivation regions in China to characterize the pathogen and to study the effect of Trichoderma spp. on L. edodes species. We identified six Trichoderma species, that is, T. harzianum, T....

The cosmetic industry is in a constant search for natural compounds or extracts with relevant bioactive properties, which became valuable ingredients to design cosmeceutical formulations. Mushrooms have been markedly studied in terms of nutritional value and medicinal properties. However, there is still slow progress in the biotechnological application of mushroom extracts in cosmetic formulati...