Aim: The present study was conducted to determine the prevalence of antibodies against leptospira using a genus specific and serovar specific antigen by indirect ELISA (iELISA) and Microscopic Agglutination test (MAT), respectively. Materials and Methods: 300 sera samples were collected from dogs, from Pondicherry, suspected to be suffering from Canine leptospirosis and with no history of vacci...

Over a period of five years (2005–2010) a total of 358 red fox (Vulpes vulpes) serum samples were collected during regular hunting procedures, mostly in the continental part of Croatia. All samples were tested using the microscopic agglutination test (MAT) for the presence of specific antibodies (Ab) to 12 Leptospira spp. pathogenic serovars. Specific antibodies for 11 different Leptospira spp....

OBJECTIVE Determining Leptospira sp. antibody seroprevalence and prevalent serovars in human and canine populations from three municipalities in the Tolima Department of Colombia. MATERIALS AND METHODS This was a cross-sectional study (subjects being selected by convenience sampling in 62 districts). Samples were collected from 850 dogs and 850 people during June, July and August 2007; these ...

A saprophytic Leptospira biflexa strain of equine origin was found which cross-reacts with immune rabbit antisera to 14 pathogenic Leptospira interrogans serotypes. Sera from goats experimentally inoculated with the saprophyte showed multiple low-level cross-agglutination reactions against a battery of live L. interrogans serotypes. Sonically treated and saline-extracted suspensions of the L. b...

A 25-years old man from Zagreb, Croatia, was admitted to the University Hospital for Infectious Diseases four days after the onset of symptoms such as fever, intense pain in the calves and anuria. The patient owned a rabbit and, before the onset of the disease, repaired some rubber pipes damaged by rodents. At admission, he had a severe clinical picture with fever, hypotension, jaundice, immobi...

OBJECTIVE To detect leptospiral antibodies by microscopic agglutination test (MAT) in north-east of Iran. METHODS This study was conducted to evaluate prevalence of human leptospiral infections by MAT, using six current reference strains of Leptospira interrogans in north-east of Iran. A total of 285 serum samples were collected from three north-east provinces of Iran, from December, 2009 to ...

This study was conducted on 210 sheep in Maku area in Iran in order to determine seroprevalence of leptospiral infection. Sera were initially screened at dilution of 1:100 against 8 live serovars of Leptospira interrogans: Pomona, Canicola, Hardjo, Ballom, Icterohaemorrhagiae, Automenalis,Australis and Grippotyphosa using the microscopic agglutination test. The prevalence of leptospiral infecti...

Leptospirosis is an important bacterial zoonotic disease that affects humans and many animal species. Knowledge of prevalence of Leptospira in a given geographic region supports the implementation of effective control/prevention programmes and thus reduces the transmission risks. This study provides Leptospira seroprevalence and serovar distribution in dogs and cats on the Caribbean island of S...

It is necessary to know the etiological structure of diseases in each region country that affects a particular animal species for successful prevention and diagnosis natural focal diseases, leptospirosis. In this paper, authors presented research materials on distribution pathogenic leptospirosis circulating populations farm animals Yakutia. Currently, 8 unfavorable points have been registered ...

BACKGROUND Previous genomic analysis of pathogenic Leptospira has identified two circular chromosomes but no plasmid. This study aims to investigate potential extrachromosomal elements of L.interrogans serovar Canicola strain Gui44. METHODOLOGY Two novel plasmids, pGui1 and pGui2, were isolated from the pathogenic strain Gui44, using a modified alkaline lysis method. Southern blotting was per...