M . A . L E G R O S ∗,‡, G . M c D E R M O T T†,‡, M . U C H I D A†,‡, C . G . K N O E C H E L ∗,‡ & C . A . L A R A B E L L ∗,†,‡ ∗Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, U.S.A. †Department of Anatomy, University of California San Francisco, San Francisco, California, U.S.A. ‡National Center for X-ray Tomography, Advanced Light Source, Berkel...

Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM)...

The presence of a probe molecule is not required in Brewster angle microscopy (BAt\1) [5,6J. This technique is based on the fact that p-polarized light is not reflected at an interface when incident under the Brewster angle, which is about 53" for the air/water interface when using Address Max-Planck-Institut fUr biophysikalische Chemie, Postfach 2841, D-37018 Gottinqen, Germany; e-mail: dmoebi...

Despite its long-standing status as the diagnostic "gold standard", the renal transplant biopsy is limited by a fundamental dependence on descriptive, empirically-derived consensus classification. The recent shift towards personalized medicine has resulted in an increased demand for precise, mechanism-based diagnoses, which is not fully met by the contemporary transplantation pathology standard...

Immunocytochemistry, the identification of cell- or tissue-bound antigens in situ, by means of a specific antibody-antigen reaction, tagged microscopically by a visible label, has a remarkably wide range of applications. The basic techniques are straightforward and can be adapted to explore the localisation of virtually any molecule of interest to the researcher in samples of normal and/or mali...

An important new method for phagocytosis research, confocal scanning fluorescence light microscopy (CSFM), is demonstrated using fluorescent microspheres ingested by murine macrophages. CSFM, in combination with Nomarski differential interference contrast microscopy (DIC), can resolve microspheres inside cells from microspheres attached to the surface of cells. Further, combined CSFM and DIC im...