نتایج جستجو برای: limiting dilution assay
تعداد نتایج: 322031 فیلتر نتایج به سال:
We have recently been able to improve the conditions for in vitro lymphocyte cultures such that every growth-inducible murine B cell, in the presence of a Bcell mitogen, will initiate growth and maturation to IgM-, IgG-, and IgAsecreting plaque-forming cells (PFC) (1, 2). 1 This was achieved mainly by adding 2-mercaptoethanol (3), a growth-supporting fetal calf serum (4), growthsupporting thymu...
FT3 values. FT3 values in the 2 patients with antibodies to T2, T3, or their conjugates were 12.3 and 32.6 pmol/L for the Vitros FT3II assay. With the Vitros FT3 assay, FT3 concentrations in 2-fold diluted control sera were 96% (1.1%) [mean (SD)] of those in the nondiluted sera, and in 2-fold diluted sera from 2 patients taking diclofenac, FT3 concentrations were 95.9% and 96.3% of those in the...
BACKGROUND Quantifying latently infected cells is critical to evaluate the efficacy of therapeutic strategies aimed at reducing the size of the long-lived viral reservoir, but the low frequency of these cells makes this very challenging. METHODS We developed TILDA (Tat/rev Induced Limiting Dilution Assay) to measure the frequency of cells with inducible multiply-spliced HIV RNA, as these tran...
ELDA is a software application for limiting dilution analysis (LDA), with particular attention to the needs of stem cell assays. It is the first limiting dilution analysis software to provide meaningful confidence intervals for all LDA data sets, including those with 0% or 100% responses. Other features include a test of the adequacy of the single-hit hypothesis, tests for frequency differences...
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