A tight junction-enriched membrane fraction has been used as immunogen to generate a monoclonal antiserum specific for this intercellular junction. Hybridomas were screened for their ability to both react on an immunoblot and localize to the junctional complex region on frozen sections of unfixed mouse liver. A stable hybridoma line has been isolated that secretes an antibody (R26.4C) that loca...

Two methods are described for the activation of cyclophosphamide by a liver microsome preparation. These procedures were applicable to an assay in vitro which tests the sensitivity of tumor cells to the drug. Satisfactory results were obtained either by pretreatment of the cyclophosphamide and removal of the microsomes before testing or by the somewhat simpler procedure of mixing drug, microsom...

We evaluated neutralizing antibodies against the Omicron variant and Anti-Spike IgG response in solid organ (SOT) or hematopoietic stem cell (HSTC) recipients after a third dose of BNT162b2 (BNT) CoronaVac (CV) following two doses CV. In total, 95 participants underwent SOT (n = 62; 44 liver, 18 kidney) HSCT 27; 5 allogeneic, 22 autologous) were included from five centers Turkey. The median tim...

background & objectives: respiratory, central nervous system, and skin complications of mustard gas toxicity have previously been studied; however, the liver and kidney side effects due to this intoxication have not been fully noted. we aimed to evaluate the frequency of liver, kidney and lung lesions in mustard gas-exposed iranian veterans who had been exposed to the toxin almost 2 decades...

background: the pathogenesis of ethanol in the liver and kidney is associated with an increase in free radicals and oxidative stress that leads to structural and functional abnormalities in these vital organs. methods: forty-eight male wistar rats were placed in eight groups and were treated as follows: control group (normal saline), group of ethanol (4 g/ kg), three doses of ardeh and honey co...

The incorporation of glucosamlneJ4C into plasma lipoproteins has been measured in the intact rat, in liver slices, and in the liver microsome fraction. The labeling of lipoprotein was studied in microsome subfractions, both in viva with glucosammeJ4C and in vitro with UDP-N-acetylglucosamineW. After isolation of lipoproteins with the aid of carrier plasma, labeled lipoproteins were found in smo...

We examined sera submitted for autoantibody testing for thyroid microsome antibodies (TMA), elevated thyroid-stimulating hormone (TSH), and free thyroxine concentrations. The frequency of TMA in antinuclear antibody-positive sera was higher (19%) than that in antinuclear antibody-negative sera (12%). Elevated TSH concentrations in serum and subnormal thyroxine concentrations in serum were assoc...

Tissue metal accumulations (gills, liver, kidney and muscle) in Persian sturgeon (Acipenser persicus) were compared folowing exposure to sublethal levels of waterborne Cd (50, 400 and 1000 μg•L-1) after periods of 1, 2, 4 and 14 days. The obtained results indicate that at the end of 4 and 14 days of exposure, total tissue cadmium concentration followed the pattern: liver > gill > kidney > muscl...