When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto complex and Kluyveromyces lodderae. Lyticase, lysing enzymes, and proteinase K appeared to be fre...

A bacteriophage, EC6, capable of lysing Escherichia coli serotype O157:H7 was isolated and found to be a member of the family Myoviridae. The genomic sequence of phage EC6 was composed of 86,231 bp with a G+C content of 38.9% and is very similar to the sequences of Escherichia phage wV8 and Salmonella phage Felix O1.

The lytic bacteriophage YS35, which is capable of lysing multidrug-resistant Pseudomonas aeruginosa strains, was isolated from a sewage sample. Here, we describe the complete genome sequence of this myoviral bacteriophage, which contains 93,296 bp of double-stranded DNA and has a 49.4% G+C content.

Phage ST0 lysing Escherichia coli H8 was isolated from wastewater and sequenced using an Illumina HiSeq system. Genomic analyses revealed that it was virulent phages and contained a circular double-stranded DNA genome, consisting of 170,496 nucleotides with an average G + C content of 37.67%. This study may provide possible alternative materials for phage therapy.

Recently, harmful algal bloom (HAB), also termed "red tide", has been recognized as a serious problem in marine environments according to climate changes worldwide. Many novel materials or methods to prevent HAB have not yet been employed except for clay dispersion, in which can the resulting sedimentation on the seafloor can also cause alteration in marine ecology or secondary environmental po...

18 An ammonium chloride erythrocytes-lysing procedure was used to prepare a bacterial pellet 19 from positive blood cultures for direct MALDI-TOF mass spectrometry analysis. 20 Identification was obtained for 78.7% of pellets. Moreover, 99% of MALDI-TOF 21 identifications were congruent at species level when considering valid scores. This fast and 22 accurate method is promising. 23

Continuous flow cell lysis by electroporation (EP), and cell and bead trapping by dielectrophoresis (DEP) have been demonstrated on a dielectrophoretic microfluidic chip. The results of lysing human white blood cells (WBC) and murine clonal cells (MN9D) by electroporation s a a m c ©

The thrombolytic (fibrinolytic) effect of the urinary plasminogen activator, urokinase (UK), was compared with its fibrinogenolytic effect, using an artificial circulating system, and in in vivo pulmonary embolus and femoral vein thrombosis models. The thrombus lysing efficiency (fibrinolysis/fibrinogenolysis) was, in all cases, below 1.00, indicating that fibrinogenolysis is a primary function...