Marchantia polymorpha L. has been established as a model liverwort species for understanding plant evolution, and several genetic transformation technologies have been developed. Recently, we described a simplified Agrobacteriummediated genetic transformation method termed AgarTrap (Agar-utilized Transformation with Pouring Solutions) that uses sporelings (S-AgarTrap) or gemmae/gemmalings (G-Ag...

To discover mechanisms that controlled the growth of the rooting system in the earliest land plants, we identified genes that control the development of rhizoids in the liverwort Marchantia polymorpha. 336,000 T-DNA transformed lines were screened for mutants with defects in rhizoid growth, and a de novo genome assembly was generated to identify the mutant genes. We report the identification of...

Previous studies of plant circadian clock evolution have often relied on models and genes defined in Arabidopsis. These identified homologues with seemingly conserved function, as well frequent gene loss. In the present study, we aimed to identify candidate liverwort Marchantia polymorpha using a more unbiased approach. To rhythm sequenced transcriptomes gemmalings time series constant light co...

Weed management is essential for sustainable crop production in all cropping systems. The use of herbicides causing the problems herbicide resistant weeds and environmental pollution. Plant-released phytotoxins have ability to promote or inhibit germination seedling growth surrounding crops weeds. Therefore, it imperative identify plant species having herbicidal potential as more safe weed appr...

The ammonium transporter gene family consists of three main clades, AMT, MEP, and Rh. The evolutionary history of the AMT/MEP/Rh gene family is characterized by multiple horizontal gene transfer events, gene family expansion and contraction, and gene loss; thus the gene tree for this family of transporters is unlike the organismal tree. The genomes of angiosperms contain genes for both electrog...

PCR-based approach was used as a phylogenetic profiling tool to probe genomic DNA samples from representatives of evolutionary distant moss taxa, namely, classes Bryopsida, Tetraphidopsida, Polytrichopsida, Andreaeopsida, and Sphagnopsida. We found relatives of all Physcomitrella patens miR390 and TAS3-like loci in these plant taxa excluding Sphagnopsida. Importantly, cloning and sequencing of ...

Presently almost no information is available on the oligosaccharide structure of the glycoproteins secreted from the methylotrophic yeast Hansenula polymorpha, a promising host for the production of recombinant proteins. In this study, we analyze the size distribution and structure of N-linked oligosaccharides attached to the recombinant glycoprotein glucose oxidase (GOD) and the cell wall mann...