نتایج جستجو برای: maldi tof mass spectrometry
تعداد نتایج: 492446 فیلتر نتایج به سال:
Bacillus are aerobic spore-forming bacteria that are known to lead to specific diseases, such as anthrax and food poisoning. This study focuses on the characterization of these bacteria by the detection of lipids extracted from 33 well-characterized strains from the Bacillus and Brevibacillus genera, with the aim to discriminate between the different species. For the purpose of analysing the li...
Identification of the causative pathogen in infectious diseases is important for surveillance and to guide treatment. In low- middle-income countries (LMIC), conventional culture identification methods, including biochemical are reference-standard. Biochemical methods can lack sensitivity specificity have slow turnaround times, causing delays definitive therapy. Matrix-assisted laser desorption...
Glycans are crucial for many key biological processes and their alterations are often a hallmark of disease. Thus, multiplexed and sensitive analysis of glycans is of intense interest. Here we report a novel approach using DNA-mediated cell surface engineering for glycan profiling by MALDI-TOF mass spectrometry (MS). Following lectin binding, DNA amplification and hybridization, glycans on the ...
This protocol describes a method for in-gel digestion of proteins after fractionation using SDS-PAGE. It is applicable to both oneand two-dimensional polyacrylamide gels of different thicknesses, acrylamide concentrations, and band (spot) sizes. This protocol followed by liquid chromatography/mass spectrometry/mass spectrometry (LC-MS/MS) or matrix-assisted laser desorption/ionization/time of f...
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