Recent developments in spatial proteomics have paved the way for retrospective in situ mass spectrometry (MS) analyses of formalin-fixed paraffin-embedded clinical tissue samples. This type of analysis is commonly referred to as matrix-assisted laser desorption/ionization (MALDI) imaging. Recently, formalin-fixed paraffin-embedded MALDI imaging analyses were augmented to allow in situ analyses ...

BACKGROUND Human papillomavirus (HPV) testing coupled with appropriate clinical management is associated with a significant decline in the rate of advanced cervical cancer and associated death. METHODS In this present study, we evaluated the performance of 2 new HPV genotyping methods, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and surface plasm...

Despite that matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has become a powerful tool in the clinical microbiology setting, few studies have till now focused on MALDI-TOF MS-based identification of dermatophytes. In this study, we analyze dermatophytes strains isolated from clinical samples by MALDI-TOF MS to supplement the reference database avai...

The levels of post-source metastable ion decay (PSD) observed in several peptides and proteins ionized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF-MS) are measured utilizing both infrared (IR) and ultraviolet (UV) desorption wavelengths. A gridless deceleration ion optic is employed to temporally separate stable analyte ions from analyte metastable...

This paper reviews the application of matrixassisted laser desorption/ionization mass spectrometry (MALDI-MS) to the analysis of synthetic polymers, and includes data obtained by Toyota CRDL. In MALDI-MS of synthetic polymers, the selection of a matrix and cationizing agent, as well as the sample preparation techniques, are very important to obtaining a successful spectrum. MALDI-MS makes it po...

MALDI-TOF MS has previously been used for imaging of large peptides and proteins embedded in tissue sections. This imaging technique is not limited to larger molecules, but can also be used to measure the presence of small molecules directly from tissue. However, for small molecule imaging the method of choice is a selected reaction monitoring approach in MS/MS mode.The benefits of parent ion i...

New developments in MALDI instrumentation, laser technology and sample preparation techniques have turned research-style MALDI imaging of proteins and peptides into a routine technique. To evaluate the potential of MALDI imaging with regard to the resolution and information content of the images that can be obtained, biological systems with well-defined structures on both the macroscopic and mi...

This study was conducted in Côte d’Ivoire to determine the respective prevalence of Candida species and their susceptibility profile antifungal agents identify emergence resistance.This retrospective from 2017 2019 at Institute Pasteur on patients with fungal disease. The yeasts isolated identified were subcultured a chromogenic medium MALDI-TOF-MS. Sensitivity tests performed using CLSI method...

BACKGROUND In Africa, relapsing fever borreliae are neglected vector-borne pathogens that cause mild to deadly septicemia and miscarriage. Screening vectors for the presence of borreliae currently requires technically demanding, time- and resource-consuming molecular methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has recently emerged as a to...

This year marks the 20th anniversary of Koichi Tanaka securing Nobel Prize in chemistry, shared with John Fenn and Kurt Wüthrich, for demonstrating applicability laser technology to analyze biological macromolecules. The principle desorption is fundamental many today’s analytical methods such as matrix-assisted desorption/ionization (MALDI). In this article, we provide an overview MALDI highlig...