Copyright © 1995 by J. Coppeta. Published by the American Institute of Aeronautics and Astronautics, Inc. with permission. Abstract In this paper a method of quantifying fluid mixing using the fluorescence intensity of fluorophors in aqueous solution is presented. The general technique involves ratioing optically separable fluorescence from two fluorescent dyes. The resulting ratio normalizes ...

bronchial asthma is the common chronic inflammatory disease and is characterized by chronic airway inflammation, airway remodeling, and airway hyperreactivity (ahr). aim of this study was to investigate the effects of mitochondrial atp-sensitive potassium channels (mitokatp) on the proliferation and secretion of human airway smooth muscle cells (hasmcs). hasmcs were treated with the serum from ...

Decoders are combinational circuits that convert information from n inputs to a maximum of 2(n) outputs. This operation is of major importance in computing systems yet it is vastly underexplored in synthetic biology. Here, we present a synthetic gene network architecture that operates as a biological decoder in human cells, converting 2 inputs to 4 outputs. As a proof-of-principle, we use small...

A set of three sucrose-regulated transcriptional fusions was constructed. Fusions p61RYTIR, p61RYlac, and p61RYice contain the scrR sucrose repressor gene and the promoterless gfp, lacZ, and inaZ reporter genes, respectively, fused to the scrY promoter from Salmonella enterica serovar Typhimurium. Cells of Erwinia herbicola containing these fusions are induced only in media amended with sucrose...

The autofluorescence intensity of bacteria and fungal spores was quantified by fluorescence microscopy in order to obtain the information for evaluating fluorescence-based bioaerosol detectors and was comparable to that of some types of polystyrene microspheres (PSMs). Although the intensity for microbes was distributed across a wide range over an order of magnitude in gray scale, it was in the...

A micro-spectroscopic method has been developed to probe the accessibility of zeolite crystals using a series of fluorescent 4-(4-diethylaminostyryl)-1-methylpyridinium iodide (DAMPI) probes of increasing molecular size. Staining large zeolite crystals with MFI (ZSM-5) topology and subsequent mapping of the resulting fluorescence using confocal fluorescence microscopy reveal differences in stru...

Submit Manuscript | http://medcraveonline.com Abbreviations: AIHA: Autoimmune Hemolytic Anemia; DIB: Double In Vivo Biotinylation; BM: Bone Marrow; PS: Phosphatidylserine; ROS: Reactive Oxygen Species; MFI: Mean Fluorescence Intensity; SEM: Standard Error of the Mean; SOP: Standard Operating Procedures; SAv-APC: StreptavidinAllophycocyanin; NHS: N-hydroxysuccinimide; 7AAD: 7-Aminoactinomycin D;...

Submit Manuscript | http://medcraveonline.com Abbreviations: CDC: Complement Dependent Cytotoxicity; FCXM: Flow-Cytometry Crossmatch; SAB: Single Antigen Bead; ESRD: End Stage Renal Disease; AMR: Antibody Mediated Rejection; DSA: Donor Specific Antibodies; MFI: Mean Fluorescence Intensity; DSA: Donor Specific Antibodies; HLA: Human Leukocyte Antigens; PRA: Panel Reactive Antibody; MCS: Median C...

Abbreviations: SCID-X1: X-linked Severe Combined Immunodeficiency; γc: Cytokine Receptor Common Gamma Chain; HSC: Hematopoietic Stem Cells; ab: Antibody; wt: Wild-type; NK cell: Natural Killer Cell; HLA: Human Leukocyte Antigen; SIN: Selfinactivating; HSV-TK: Thymidine kinase of Herpes Simplex Virus; ER: Endoplasmatic Reticulum; MFI: Mean Fluorescence Intensity; CDC: Complement-dependent Cytoto...