Survival rates of Ascosphaera aggregata and Ascosphaera apis over the course of a year were tested using different storage treatments. For spores, the storage methods tested were freeze-drying and ultra-low temperatures, and for hyphae, freeze-drying, agar slants, and two methods of ultra-low temperatures. Spores of A. aggregata and A. apis stored well at -80 degrees C and after freeze-drying. ...

inject liquid formulation technology (1), the need arose for a process whereby large quantities of highly polished spherical glass microspheres of known density could be manufactured. The process needed to be inexpensive, scalable, GMP-compliant and capable of sterile manufacture for parenteral drugs. Methods such as emulsion drying, freeze drying and spray-freeze drying were all investigated, ...

In prospect of developing an oral dosage form of Infliximab, for treatment of Crohn's disease and rheumatoid arthritis, freeze-drying (vial vs Lyoguard trays) and spray-drying were investigated as production method for stable powders. Dextran and inulin were used in combination with sucrose as stabilizing excipients. The drying processes did not affect Infliximab in these formulations, i.e. bot...

Freeze-dried mouse spermatozoa can be used for normal embryonic development after injection into oocytes, thus indicating that freeze-drying is a useful method for the storage and transportation of genetic materials from animals. We recently reported that storage of freeze-dried mouse spermatozoa requires maintenance at temperatures lower than -80 C for long-term preservation and a pressure of ...

conclusions although quince seeds dried mucilage by lyophilization had less yield amounts yet in view of purity and physicochemical and mechanical properties, it was significantly better than mucilage prepared by the oven method (p < 0.05). results the yield value of mucilage from the warm method was about three folds greater than the amount from the cold method. the phytochemical, physicochemi...

Coupling an infrared (IR) camera to a freeze dryer for on-line monitoring of freeze-drying cycles is described for the first time. Normally, product temperature is measured using a few invasive Pt-100 probes, resulting in poor spatial resolution. To overcome this, an IR camera was placed on a process-scale freeze dryer. Imaging took place every 120 s through a Germanium window comprising 30,000...

Chitosan-pectin, chitosan-alginate and chitosan-pectin-alginate scaffolds were prepared by freeze-drying technique. The physicochemical characteristics of the scaffolds were characterized by FTIR and SEM. The biological activities like antibacterial assessment, swelling behavior, biodegradation and cytotoxicity study of the prepared scaffolds were evaluated. The results show high swelling prope...

Freeze-dried spermatozoa are capable of participating in normal embryonic development after injection into oocytes and thus useful for the maintenance of genetic materials. We recently reported that long-term preservation of freeze-dried mouse spermatozoa by conventional methods requires temperatures lower than -80 degrees C. Successful permanent preservation of mouse spermatozoa at much higher...

The present investigation represents the effect of freeze drying on some properties as acid and bile tolerance of Streptococcus thermophilus MTCC 1938 culture isolated from dairy products. The cell paste obtained from milk based medium was freeze dried with a pressure of 50-100 mtorr for 24h at -40°C. Acid and bile tolerance test exhibited 3.8-4.9 and 3.2-3.8 log counts reduction after freeze d...

the main purpose of the present study is to investigate the effect of air drying, freeze drying and vacuum drying methods on viscosity and gelation of lipidium perfoliatum seed gum. results showed that the viscosity values of samples varied from 0.31 to 1.26 pa.s at (shear rate = 12.23 s-1) and control sample gum exhibited the highest viscosity. apparent viscosity was decreased by all of drying...