نتایج جستجو برای: melting curve analysis mca

تعداد نتایج: 2935542  

Journal: :Clinical chemistry 2005
Elaine Lyon

Two reports in this issue of Clinical Chemistry describe novel mutations in thrombophilia genes (1, 2). The authors of the reports describe variants detected by the identification of a melting temperature (Tm) different from the expected Tm of the target mutation and from the Tm of the wild-type sequence. The variants reported in this issue are A20218G in the prothrombin (factor II) gene and C1...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2008
Daisuke Takaiwa Itaru Hatano Kenichiro Koga Hideki Tanaka

A phase diagram of water in single-walled carbon nanotubes at atmospheric pressure is proposed, which summarizes ice structures and their melting points as a function of the tube diameter up to 1.7 nm. The investigation is based on extensive molecular dynamics simulations over numerous thermodynamic states on the temperature-diameter plane. Spontaneous freezing of water in the simulations and t...

Journal: :Stroke 2010
Robert Mikulik Ladislav Dusek Michael D Hill Eva Fulep James C Grotta Marc Ribo Carlos Molina Andrei V Alexandrov

BACKGROUND AND PURPOSE Early recanalization is the likely mechanism by which intravenous thrombolysis improves stroke outcomes. Limited data exist on the patterns of early recovery of various brain functions. METHODS Data from the Combined Lysis of Thrombus in Brain Ischemia Using Transcranial Ultrasound and Systemic t-PA (CLOTBUST) trial was used to determine time-related trends in neurologi...

Journal: :BioTechniques 2001
J M Akey D Sosnoski E Parra S Dios K Hiester B Su C Bonilla L Jin M D Shriver

High-throughput methods for assaying DNA variation require two important steps: (i) discriminating the variation and (ii) detecting the signal. In this report, we describe a novel SNP genotyping method that we refer to as melting curve analysis of SNPs (McSNP). McSNP combines a classic approach for discriminating alleles, restriction enzyme digestion, with a more recent method for detecting DNA...

Journal: :applied biotechnology reports 0
mohammad najarasl mohammad sadegh hashemzadeh bentolhoda zahraei samaneh zahiri yeganeh1 mahdi tat mojtaba sharti

influenza a virus is now considered to be widespread in poultry and has demonstrated the ability to infect humans in iran. for laboratory diagnosis of these respiratory viruses, it is essential to have rapid methods, able to detect viruses in early stages of the infection in clinical specimens. the real-time reverse-transcription polymerase chain reaction n (rrt-pcr) assay has been established ...

Journal: :Clinical chemistry 2007
Joshua G Vandersteen Pinar Bayrak-Toydemir Robert A Palais Carl T Wittwer

BACKGROUND Heteroduplex scanning techniques usually detect all heterozygotes, including common variants not of clinical interest. METHODS We conducted high-resolution melting analysis on the 24 exons of the ACVRL1 and ENG genes implicated in hereditary hemorrhagic telangiectasia (HHT). DNA in samples from 13 controls and 19 patients was PCR amplified in the presence of LCGreen I, and all 768 ...

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