the western flower thrips frankliniella occidentalis, an important greenhouse pest, has acquired rapid resistance to the chemical pesticides. therefore, biological control is worth consideration as an alternative control method. among the biological control agents, entomopathogenic fungi showed to be quite successful in some occasions. in this study, three iranian isolates of metarhizium anisop...

We present two cases of keratitis due to Metarhizium anisopliae in geographically separated areas of the United States. The isolates were microscopically similar but morphologically different and were identified by ribosomal DNA sequencing. Both isolates had low minimum inhibitory concentration (MIC) values to caspofungin and micafungin, but high MIC values to amphotericin B. The morphologic an...

Fungus-farming ant colonies vary four to five orders of magnitude in size. They employ compounds from actinomycete bacteria and exocrine glands as antimicrobial agents. Atta colonies have millions of ants and are particularly relevant for understanding hygienic strategies as they have abandoned their ancestors' prime dependence on antibiotic-based biological control in favour of using metapleur...

Entomopathogenic fungi are naturally occurring pathogens of some insects that produce enzymes capable of penetrating into an insect’s cuticle. Chitinases are thought to be responsible for entomopathogenic fungi’s ability to degrade cuticle. Primers were designed for the chi3 gene which encodes a chitinase CHIT30, from the mRNA transcript present in Metarhizium anisopliae. These primers were una...

The aim of the study was to determine effect copper application in combination with organic materials on genus composition and intensification occurrence entomopathogenic fungi soil. three-year pot experiment carried out greenhouse conditions. set up a completely random design, taking into account two factors: dose fertilization. Soil samples for tests were collected third year experiment, spri...

Metarhizium robertsii is a plant root colonizing fungus that is also an insect pathogen. Its entomopathogenicity is a characteristic that was acquired during evolution from a plant endophyte ancestor. This transition provides a novel perspective on how new functional mechanisms important for host switching and virulence have evolved. From a random T-DNA insertion library, we obtained a pathogen...

BACKGROUND The protective effect of two isolates of an entomopathogenic fungus, Metarhizium anisopliae (DEMI 002 and Iran 437C) on the adult stage of Varroa destructor was evaluated in comparison with fluvalinate strips in the field. METHODS A total of 12 honey bee colonies were provided from an apiculture farm. The selected hives were divided into 4 groups (3 hives per group). The first grou...

Liu S.-F., Wang G.-J., Nong X.-Q., Liu B., Wang M.-M., Li S.-L., Cao G.-C., Zhang Z.-H. (2017): Entomopathogen Metarhizium anisopliae promotes the early development of peanut root. Plant Protect. Sci., 53: 101–107. The benefit of the entomopathogen Metarhizium anisopliae to early root development was evaluated. Two inoculating methods, conidia-suspension-drenching (T1) and conidia-coating (T2),...

The low survival of microbial pest control agents exposed to UV is the major environmental factor limiting their effectiveness. Using gene disruption we demonstrated that the insect pathogenic fungus Metarhizium robertsii uses photolyases to remove UV-induced cyclobutane pyrimidine dimers (CPD) and pyrimidine (6-4) photoproducts [(6-4)PPs] from its DNA. However, this photorepair is insufficient...

Fungal entomopathogens rely on cellular heterogeneity during the different stages of insect host infection. Their pathogenicity is exhibited through the secretion of secondary metabolites, which implies that the infection life history of this group of environmentally important fungi can be revealed using metabolomics. Here metabolomic analysis in combination with ex vivo insect tissue culturing...