materials and methods total protein was determined by lowery method. bovine lens alr2 was partially purified by gel filtration chromatography on sephadextm g25. bovine cortex kidney alr1 was partially purified by diethylaminoethyl (deae) precipitation. the hydroalcoholic extract was obtained from frozen propolis. the enzyme activity and sorbitol accumulation in erythrocytes were determined spec...

The objective of the present study was to determine whether β1-4 galacto-oligosaccharides (GOS) and Candida kefyr combined with nitrate as manipulators could suppress rumen methanogenesis without nitrate poisoning in sheep. Four rumen fistulated wethers were allocated to a 4×4 Latin square design. Nitrate (1.3 g NaNO3 kg body weight) with and without GOS and Candida kefyr were administered into...

Soot is a mass of impure particles carbon obtained from incomplete hydrocarbon combustion. an ultrafine air-borne pollutant and enters into the body through ingestion, skin contact inhalation causing devastating effects on blood cells. The aim study was to determine effect soot methaemoglobin oxyhaemoglobin levels individuals resident in both Iwofe, Port Harcourt (exposed subjects) Ihiala, Anam...

The time course of haemoglobin autoxidation was studied under various conditions at 37 degrees C, and the changes in oxyhaemoglobin, intermediate haemoglobins and methaemoglobin during the reaction were analysed by isoelectric focusing on Ampholine/polyacrylamide-gel plates. Under various conditions (10 mM-phosphate buffer, 10 mM-phosphate buffer with 0.1 M-phosphate buffer, 10 mM-phosphate buf...

We report a case of fatal methaemoglobinaema resulting from sodium nitrite poisoning. A 28 year old woman arrested in the emergency department following collapse. During resuscitation venous blood gas revealed methaemoglobin percentage 81%. Following treatment with methylene blue, bicarbonate and adrenaline, decreased. Prior to transfer intensive care, CT head extensive hypoxic brain injury. Tw...

Sodium nitrite is a strong oxidising agent with multiple industrial uses. Ingestion of small dose can cause death rapidly through the formation methaemoglobin and nitric oxide. Globally, while deaths due to sodium ingestion have been sporadic tended be accidental, frequency purposeful ingestions increasing. Local incidences in Singapore remain rare. We present case report Singapore, challenges ...