Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoterswhich are regulated by methanol. However, the use of methanol has several disadvantages,which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) arefocusing on methanol mixed feeding strategies. This work aimed to develop a new experimentalmethod and compare the effect of vario...

The effects of extracting solvents (dichloromethane, ethyl acetate and methanol) on the valerenic acid content and antioxidant activity of roots extracts of Valeriana jatamansi (cultivated and wild) were investigated. The tested plant extracts contained appreciable amounts of valerenic acid content which affects the antioxidant potential of plant extracts. Higher concentration of valerenic acid...

methanol is responsible for a life-threatening poisoning. fomepizole, a potent alcohol dehydrogenase inhibitor, is an efficient and safe antidote that prevents or reduces toxic methanol metabolism. although no study has compared its efficacy with ethanol, fomepizole is recommended as a first-line antidote. treatment should be started as soon as possible, based on history and initial findings in...

The aim of this study was to evaluate the anti-oxidant activity of the flowers of the plant Vernonia cinerea (Family: Asteraceae). Flowers extracts of V. cinerea in different four organic solvents (methanol, hexane, chloroform and ethyl acetate) and water were assayed for antioxidant activity using FRAP assay. All of these extracts were found to possess significant antioxidant activity at 5mg/m...

1. Abstract Pt-Ru/C catalyst has been applied extensively in direct methanol fuel cells for methanol electrochemical oxidation. In this study, a model for electrochemical kinetics of methanol has been developed for electrocatalyst Pt-Ru/C. The single cell performance of DMFC and AC impedance analysis were measured. The methanol crossover problem still remains a challenge for the applications of...

The structural organization in mixtures of 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF(4)])/water or methanol was studied by infrared spectroscopy. No drastic change in the concentration dependence of the alkyl C-H band frequency was observed at high concentration of the ionic liquid. This behavior indicates a clustering of the ionic liquid in alkyl regions. Nevertheless, the prese...

The effects of various parameters on bromate reduction were tested using lab-scale batch reactors with sulfur based autotrophic and methanol based heterotrophic denitrification processes. The initial bromate (BrO3-) concentration of 100 and 500 μg/L was completely reduced and bromide (Br-) was produced stoichiometrically from bromate in all batch reactors. In all experiments, nitrate was comple...

Raman spectra of Carbon Tetrachloride and Methanol solutions were measured. Spectral lines ν3,νcom(ν1+ν4) of Carbon Tetrachloride Vibrational Spectra were analyzed. The results showed that Fermi resonance coefficients depend on methanol concentration, besides to the existence of clear effect for the polarization of carbon on these coefficients. In addition, cases of splitting in the energy leve...

Cell morphology is the research foundation in many applications related to the estimation of cell status, drug response, and toxicity screening. In biomedical field, the quantitative phase detection is an inevitable trend for living cells. In this paper, the morphological change of HeLa cells treated with methanol of different concentrations is detected using digital holographic microscopy. The...

background consumption of home-distilled alcohol may lead to epidemic or sporadic cases of severe acute methanol poisoning. the difficulty of establishing strict indications for hemodialysis in acute methanol poisoning is a widely recognized issue. materials and methods the determination of the clinical, especially hemodialysis, and para clinical factors influencing patient survival in 46 acute...